靶向闭塞小鼠皮质的单个软脑膜血管。
Targeted Occlusion of Individual Pial Vessels of Mouse Cortex.
作者信息
Taylor Zachary J, Shih Andy Y
机构信息
Department of Neurosciences, Medical University of South Carolina, Charleston, USA.
出版信息
Bio Protoc. 2013 Sep 5;3(17). doi: 10.21769/bioprotoc.897.
Targeted photothrombosis is a method to occlude individual arterioles and venules that lie on the surface of the cerebral cortex. It has been used to study collateral flow patterns within the pial vascular network following occlusion of single surface vessels (Schaffer ., 2006; Blinder ., 2010; Nguyen ., 2011), as well as to generate localized ischemic strokes following occlusion of single penetrating vessels (Nishimura ., 2007; Drew ., 2010; Shih ., 2013). The intravascular clot is formed by irradiation of a target vessel with a focused green laser after injection of a circulating photosensitizing agent, Rose Bengal (Watson ., 1985). We briefly describe modifications of custom-designed and commercial two-photon imaging systems required to introduce a green laser for photothrombosis. We further provide instructions on how to occlude a single penetrating arteriole within the somatosensory cortex of an anesthetized mouse.
靶向光血栓形成是一种闭塞位于大脑皮质表面的单个小动脉和小静脉的方法。它已被用于研究单个表面血管闭塞后软脑膜血管网络内的侧支血流模式（Schaffer等，2006年；Blinder等，2010年；Nguyen等，2011年），以及在单个穿通血管闭塞后产生局部缺血性中风（Nishimura等，2007年；Drew等，2010年；Shih等，2013年）。在注射循环光敏剂孟加拉玫瑰红后，用聚焦绿色激光照射目标血管，形成血管内血栓（Watson等，1985年）。我们简要描述了为引入用于光血栓形成的绿色激光而对定制设计和商用双光子成像系统进行的修改。我们还进一步提供了如何在麻醉小鼠的体感皮质内闭塞单个穿通小动脉的操作说明。
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