Alsemgeest Jenifer, Old Julie M, Young Lauren J
School of Medical and Applied Sciences, Central Queensland University, Rockhampton, Queensland 4702, Australia.
School of Science and Health, Western Sydney University, Hawkesbury, Penrith, NSW 2751, Australia.
Cytokine. 2016 Dec;88:37-44. doi: 10.1016/j.cyto.2016.08.012. Epub 2016 Aug 20.
Interleukin-10 is an immunomodulatory cytokine that has been implicated, along with IFN-γ, in the disease sequelae of mycobacterial infection. In order to investigate the role of IL-10 in marsupial disease models we sequenced and characterised the IL10 gene in the tammar wallaby (Macropus eugenii) and rufous hare-wallaby (Lagorchestes hirsutus). An isoform IL-10Δ3, in which an in-frame deletion of exon 3 occurs, was discovered in both macropod species. Analysis of wallaby and other reported marsupial IL-10 homologs suggests that while marsupial IL-10 is comparable to that of human IL-10, the predicted IL-10Δ3 protein may play a more complicated role in the modulation of IL-10-directed responses. Expression of the canonical gene and splicing variant was confirmed in both wallabies, and the rufous hare-wallaby showed differential expression across lymph node, spleen and liver, with isoform expression detected in the lymph node. This characterisation and expression of IL-10 in de novo tissues provides a basis for further study into the role of IL-10 in disease models in marsupials.
白细胞介素-10是一种免疫调节细胞因子，与干扰素-γ一起，参与了分枝杆菌感染的疾病后遗症。为了研究白细胞介素-10在有袋动物疾病模型中的作用，我们对帚尾袋鼩（Macropus eugenii）和赤褐兔袋鼠（Lagorchestes hirsutus）的IL10基因进行了测序和特征分析。在这两种有袋类动物中都发现了一种异构体IL-10Δ3，其中外显子3发生了框内缺失。对袋鼩和其他已报道的有袋动物白细胞介素-10同源物的分析表明，虽然有袋动物的白细胞介素-10与人类白细胞介素-10相当，但预测的IL-10Δ3蛋白可能在白细胞介素-10介导的反应调节中发挥更复杂的作用。在两种袋鼩中都证实了典型基因和剪接变体的表达，赤褐兔袋鼠在淋巴结、脾脏和肝脏中表现出差异表达，在淋巴结中检测到异构体表达。这种在新组织中白细胞介素-10的特征和表达为进一步研究白细胞介素-10在有袋动物疾病模型中的作用提供了基础。
