牙源性角化囊性瘤过度表达与侵袭性伪足相关的蛋白，提示侵袭性伪足的形成。

Keratocystic odontogenic tumor overexpresses invadopodia-related proteins, suggesting invadopodia formation.

作者信息

Ribeiro Ribeiro André Luis, da Costa Natacha Malu Miranda, de Siqueira Adriane Sousa, Brasil da Silva Walessa, da Silva Kataoka Maria Sueli, Jaeger Ruy Gastaldoni, de Melo Alves-Junior Sérgio, Smith Andrew M, de Jesus Viana Pinheiro João

机构信息

Department of Oral and Maxillofacial Surgery, School of Dentistry, University Center of Pará - CESUPA, Belém, Brazil; Department of Microbial Diseases, Eastman Dental Institute, University College London, London, England.

Cell Culture Laboratory, Faculty of Dentistry, Federal University of Pará, Belém, Brazil.

出版信息

Oral Surg Oral Med Oral Pathol Oral Radiol. 2016 Oct;122(4):500-8. doi: 10.1016/j.oooo.2016.06.004. Epub 2016 Jun 22.

DOI:10.1016/j.oooo.2016.06.004

PMID:27554376

Abstract

OBJECTIVE

Keratocystic odontogenic tumor (KOT) is an odontogenic neoplasm that shows aggressive clinical behavior and local invasiveness. Invadopodia are actin-rich cellular protrusions exhibiting proteolytic pericellular activity, thereby inducing focal invasion in neoplastic cells and increasing neoplasms aggressiveness. Thus, this study aimed to evaluate immunoexpression of invadopodia-related proteins, cortactin, MT1-MMP, Tks4, and Tks5, in KOT.

STUDY DESIGN

Immunohistochemistry of 16 cases of KOT, eight cases of calcifying cystic odontogenic tumor (CCOT), and eight samples of the oral mucosa (OM) was carried out to assess the expression of the above described invadopodia-related proteins in the basal and suprabasal layer.

RESULTS

KOT samples showed higher and significant immunoexpression of cortactin, MT1-MMP, TKs4, and TKs5 compared with the CCOT and OM samples. Significant expression of all these proteins was observed in the basal layer compared with the suprabasal layer in KOT.

CONCLUSIONS

Overexpression of cortactin, MT1-MMP, TKs4, and TKs5 was observed in KOT compared with samples of CCOT and OM. These proteins were also overexpressed in the basal over the suprabasal layer of KOT samples. Taken together, these results suggest the participation of invadopodia-related proteins on the pathogenesis of this lesion.

摘要

目的

牙源性角化囊性瘤（KOT）是一种具有侵袭性临床行为和局部侵袭性的牙源性肿瘤。侵袭伪足是富含肌动蛋白的细胞突起，表现出细胞周围蛋白水解活性，从而诱导肿瘤细胞的局部侵袭并增加肿瘤的侵袭性。因此，本研究旨在评估侵袭伪足相关蛋白、皮层肌动蛋白、基质金属蛋白酶-1（MT1-MMP）、Tks4和Tks5在牙源性角化囊性瘤中的免疫表达。