Ribeiro Ribeiro André Luis, da Costa Natacha Malu Miranda, de Siqueira Adriane Sousa, Brasil da Silva Walessa, da Silva Kataoka Maria Sueli, Jaeger Ruy Gastaldoni, de Melo Alves-Junior Sérgio, Smith Andrew M, de Jesus Viana Pinheiro João
Department of Oral and Maxillofacial Surgery, School of Dentistry, University Center of Pará - CESUPA, Belém, Brazil; Department of Microbial Diseases, Eastman Dental Institute, University College London, London, England.
Cell Culture Laboratory, Faculty of Dentistry, Federal University of Pará, Belém, Brazil.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2016 Oct;122(4):500-8. doi: 10.1016/j.oooo.2016.06.004. Epub 2016 Jun 22.
Keratocystic odontogenic tumor (KOT) is an odontogenic neoplasm that shows aggressive clinical behavior and local invasiveness. Invadopodia are actin-rich cellular protrusions exhibiting proteolytic pericellular activity, thereby inducing focal invasion in neoplastic cells and increasing neoplasms aggressiveness. Thus, this study aimed to evaluate immunoexpression of invadopodia-related proteins, cortactin, MT1-MMP, Tks4, and Tks5, in KOT.
Immunohistochemistry of 16 cases of KOT, eight cases of calcifying cystic odontogenic tumor (CCOT), and eight samples of the oral mucosa (OM) was carried out to assess the expression of the above described invadopodia-related proteins in the basal and suprabasal layer.
KOT samples showed higher and significant immunoexpression of cortactin, MT1-MMP, TKs4, and TKs5 compared with the CCOT and OM samples. Significant expression of all these proteins was observed in the basal layer compared with the suprabasal layer in KOT.
Overexpression of cortactin, MT1-MMP, TKs4, and TKs5 was observed in KOT compared with samples of CCOT and OM. These proteins were also overexpressed in the basal over the suprabasal layer of KOT samples. Taken together, these results suggest the participation of invadopodia-related proteins on the pathogenesis of this lesion.
牙源性角化囊性瘤（KOT）是一种具有侵袭性临床行为和局部侵袭性的牙源性肿瘤。侵袭伪足是富含肌动蛋白的细胞突起，表现出细胞周围蛋白水解活性，从而诱导肿瘤细胞的局部侵袭并增加肿瘤的侵袭性。因此，本研究旨在评估侵袭伪足相关蛋白、皮层肌动蛋白、基质金属蛋白酶-1（MT1-MMP）、Tks4和Tks5在牙源性角化囊性瘤中的免疫表达。
对16例牙源性角化囊性瘤、8例牙源性钙化囊性瘤（CCOT）和8例口腔黏膜（OM）样本进行免疫组织化学检测，以评估上述侵袭伪足相关蛋白在基底层和基底上层的表达。
与牙源性钙化囊性瘤和口腔黏膜样本相比，牙源性角化囊性瘤样本中皮层肌动蛋白、MT1-MMP、Tks4和Tks5的免疫表达更高且具有显著性。在牙源性角化囊性瘤中，与基底上层相比，所有这些蛋白在基底层均有显著表达。
与牙源性钙化囊性瘤和口腔黏膜样本相比，牙源性角化囊性瘤中观察到皮层肌动蛋白、MT1-MMP、Tks4和Tks5的过表达。这些蛋白在牙源性角化囊性瘤样本的基底层也比基底上层过表达。综上所述，这些结果表明侵袭伪足相关蛋白参与了该病变的发病机制。
