Low K G, Nielsen C P, West N B, Douglass J, Brenner R M, Maslar I A, Melner M H
Division of Reproductive Biology and Behavior, Oregon Regional Primate Research Center, Beaverton 97006.
Mol Endocrinol. 1989 May;3(5):852-7. doi: 10.1210/mend-3-5-852.
Proenkephalin mRNA has previously been shown to be expressed in the rodent uterus with varying levels during the estrous cycle. To examine for the potential regulation of proenkephalin gene expression by steroid hormones in a primate displaying a menstrual cycle and to define the functional tissue within the uterus expressing this transcript, we have used Northern blot analysis of extracted RNA from isolated uterine tissue subtypes from normal adult rhesus macaques obtained during the menstrual cycle and from ovariectomized females under different physiological steroid hormone treatments. A strong band of proenkephalin mRNA of 1.3 kilobases was detected almost exclusively in the proliferative endometrium from monkeys in the follicular phase of the cycle. No proenkephalin mRNA was detected in secretory endometrium obtained from monkeys in the luteal phase. When ovariectomized macaques were implanted with silastic capsules of 17 beta-estradiol, proenkephalin mRNA was detected in the endometrium but not the myometrium of the estradiol-treated animals. No proenkephalin mRNA was detected in ovariectomized control animals. Under these conditions, we were unable to detect proenkephalin mRNA in ovariectomized macaques implanted with separate silastic capsules of 17 beta-estradiol and progesterone or in decidual tissue from early or late pregnancy. These results suggest that in the primate uterus 1) proenkephalin mRNA is expressed primarily in the endometrium of the uterus, 2) expression of the proenkephalin gene is regulated by 17 beta-estradiol in the endometrium, and 3) this effect of estradiol is antagonized by progesterone.
前脑啡肽原mRNA此前已被证明在啮齿动物子宫中表达，且在发情周期中水平各异。为了研究类固醇激素对具有月经周期的灵长类动物前脑啡肽原基因表达的潜在调节作用，并确定子宫内表达该转录本的功能组织，我们对从正常成年恒河猴月经周期中获取的分离子宫组织亚型以及不同生理类固醇激素处理下的去卵巢雌性动物提取的RNA进行了Northern印迹分析。在周期卵泡期猴子的增殖期子宫内膜中几乎只检测到一条1.3千碱基的强前脑啡肽原mRNA条带。在黄体期猴子的分泌期子宫内膜中未检测到前脑啡肽原mRNA。当给去卵巢的猕猴植入17β - 雌二醇的硅橡胶胶囊时，在接受雌二醇处理动物的子宫内膜中检测到了前脑啡肽原mRNA，但在肌层中未检测到。在去卵巢对照动物中未检测到前脑啡肽原mRNA。在这些条件下，我们在植入单独的17β - 雌二醇和孕酮硅橡胶胶囊的去卵巢猕猴或妊娠早期或晚期的蜕膜组织中未能检测到前脑啡肽原mRNA。这些结果表明，在灵长类动物子宫中：1）前脑啡肽原mRNA主要在子宫内膜中表达；2）前脑啡肽原基因的表达在子宫内膜中受17β - 雌二醇调节；3）雌二醇的这种作用被孕酮拮抗。
