Zurawski V R, Novotný J, Haber E, Margolies M N
J Immunol. 1978 Jul;121(1):122-9.
Intravenous injection of New Zealand White rabbits with type III pneumococcal polysaccharide vaccine conjugated with the cardiac glycoside digoxin resulted in the production of both antidigoxin and anti-type III pneumococcal polysacharide antibodies. Among antisera of 12 rabbits examined during their peak antibody production periods, 1 to 20 mg (mean, 5.4 mg) of antidigoxin antibody could be recovered from 1 ml of serum. Antisera from five of these 12 rabbits contained antidigoxin antibodies of restricted heterogeneity as demonstrated by urea-polyacrylamide disc gel electrophoresis of fully reduced and alkylated antibodies. From the antisera of four of these five rabbits, electrophoretically homogeneous antibodies (1 to 5 mg/ml antiserum) could be isolated by affinity chromatography on ouabain-amine-Sepharose columns. The structural homogeneity of two of these antidigoxin antibodies was confirmed by amino acid sequence analysis of purified light chains through the first hypervariable region. These data suggest that the conjugation of small molecules to bacterial polysaccharide vaccines may provide a general method for synthesis of immunogens that can regularly elicit antihapten antibodies of restricted heterogeneity.
给新西兰白兔静脉注射与强心苷地高辛偶联的Ⅲ型肺炎球菌多糖疫苗，可产生抗地高辛抗体和抗Ⅲ型肺炎球菌多糖抗体。在12只兔子抗体产生高峰期检测的抗血清中，每1毫升血清可回收1至20毫克（平均5.4毫克）抗地高辛抗体。通过对完全还原和烷基化抗体进行尿素-聚丙烯酰胺圆盘凝胶电泳表明，这12只兔子中有5只的抗血清含有异质性受限的抗地高辛抗体。从这5只兔子中的4只的抗血清中，通过哇巴因-胺-琼脂糖柱亲和层析可分离出电泳纯的抗体（每毫升抗血清1至5毫克）。通过对纯化轻链直至第一个高变区进行氨基酸序列分析，证实了其中两种抗地高辛抗体的结构同质性。这些数据表明，小分子与细菌多糖疫苗的偶联可能为合成免疫原提供一种通用方法，这种免疫原可定期引发异质性受限的抗半抗原抗体。
