Charyasriwong Siriwan, Haruyama Takahiro, Kobayashi Nobuyuki
Laboratory of Molecular Biology of Infectious Agents, Graduate School of Biomedical Sciences, Nagasaki University.
Drug Discov Ther. 2016;10(4):201-10. doi: 10.5582/ddt.2016.01045. Epub 2016 Aug 23.
Influenza A and B virus infections are serious public health concerns globally. However, the concerns regarding influenza B infection have been underestimated. The currently used anti-influenza drugs have not provided equal efficacy for both influenza A and B viruses. Susceptibility to neuraminidase (NA) inhibitors has been observed to be lower for influenza B viruses than for influenza A viruses. Moreover, the emergence of resistance to anti-influenza drugs underscores the need to develop new drugs. Recently, we reported that methylglyoxal (MGO) suppressed influenza A virus replication in a strain-independent manner. Therefore, we hypothesize that MGO exhibits anti-influenza activity against B strains. This study aimed to evaluate the anti-influenza viral activity of MGO against influenza B strains by using Madin-Darby canine kidney (MDCK) cells. Several types of influenza B viruses were used to determine the activity of MGO. The susceptibilities of influenza A and B viruses to NA inhibitors were compared. MGO inhibited influenza B virus replication, with 50% inhibitory concentrations ranging from 23-140 μM, which indicated greater sensitivity of influenza B viruses than influenza A viruses. Our results show that MGO has potent inhibitory activity against influenza B viruses, including NA inhibitor-resistant strains.
甲型和乙型流感病毒感染是全球严重的公共卫生问题。然而，对乙型流感感染的关注一直被低估。目前使用的抗流感药物对甲型和乙型流感病毒的疗效并不相同。据观察，乙型流感病毒对神经氨酸酶（NA）抑制剂的敏感性低于甲型流感病毒。此外，抗流感药物耐药性的出现凸显了开发新药的必要性。最近，我们报道了甲基乙二醛（MGO）以菌株独立的方式抑制甲型流感病毒复制。因此，我们假设MGO对乙型流感病毒株具有抗流感活性。本研究旨在通过使用犬肾传代细胞（MDCK）来评估MGO对乙型流感病毒株的抗流感病毒活性。使用了几种乙型流感病毒来测定MGO的活性。比较了甲型和乙型流感病毒对NA抑制剂的敏感性。MGO抑制乙型流感病毒复制，50%抑制浓度范围为23 - 140μM，这表明乙型流感病毒比甲型流感病毒更敏感。我们的结果表明，MGO对乙型流感病毒具有强大的抑制活性，包括对NA抑制剂耐药的毒株。
