Chandrasekaran Abinaya, Varga Eszter, Nemes Csilla, Táncos Zsuzsanna, Kobolák Julianna, Dinnyés András
BioTalentum Ltd., Gödöllő, Hungary.
BioTalentum Ltd., Gödöllő, Hungary; Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllő, Hungary.
Stem Cell Res. 2016 Jul;17(1):78-80. doi: 10.1016/j.scr.2016.05.014. Epub 2016 May 24.
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterised 63-year old woman with late onset Alzheimer's disease (LOAD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously towards the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to further study the pathomechanism of sporadic AD, to identify early biomarkers and also for drug testing and gene therapy studies.
从一名临床诊断为迟发性阿尔茨海默病（LOAD）的63岁女性患者身上采集外周血单核细胞（PBMCs）。使用仙台病毒递送系统，用人OSKM转录因子对PBMCs进行重编程。通过免疫细胞化学检测多能性标志物，证实无转基因的诱导多能干细胞（iPSC）具有多能性，并在体外自发分化为三个胚层。此外，该iPSC系显示出正常的核型。我们的模型可能为进一步研究散发性阿尔茨海默病的发病机制、识别早期生物标志物以及进行药物测试和基因治疗研究提供一个良好的平台。
