Xu Pan, Wang Kezhu, Lu Cong, Dong Liming, Gao Li, Yan Ming, Aibai Silafu, Liu Xinmin
Research Center of Pharmacology and Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
Department of Pharmacology and Toxicology Laboratory, Xinjiang Institute of Traditional Uighur Medicine, Urumqi, Xinjiang 830049, China.
J Ethnopharmacol. 2016 Dec 4;193:408-415. doi: 10.1016/j.jep.2016.08.030. Epub 2016 Aug 21.
Lavender essential oil (LO), an aromatic liquid extracted from Lavandula angustifolia Mill., has been traditionally used in the treatments of many nervous system diseases, and recently LO also reported to be effective for the Alzheimer's disease (AD).
The improvement effect of lavender oil (LO) on the scopolamine-induced cognitive deficits in mice and HO induced cytotoxicity in PC12 cells have been evaluated. The relevant mechanism was also researched from the perspective of antioxidant effect and cholinergic system modulation.
Cognitive deficits were induced in C57BL/6J mice treated with scopolamine (1mg/kg, i.p.) and were assessed by Morris water maze (MWM) and step-through passive avoidance tests. Then their hippocampus were removed for biochemical assays (acetylcholinesterase (AChE), superoxide dismutase (SOD), glutathione peroxidase (GPX) and malondialdehyde (MDA)). In vitro, the cytotoxicity were induced by 4h exposure to HO in PC12 and evaluated by cell viability (MTT), lactate dehydrogenase (LDH) level, nitric oxide (NO) release, reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP).
The results demonstrated that LO (100mg/kg) could improve the cognitive performance of scopolamine induced mice in behavioral tests. Meanwhile, it significantly decreased the AChE activity, MDA level, and increase SOD and GPX activities of the model. Moreover, LO (12μg/mL) protected PC12 cells from HO induced cytotoxicity by reducing LDH, NO release, intracellular ROS accumulation and MMP loss.
It was suggested that LO could show neuroprotective effect in AD model in vivo (scopolamine-treated mice) and in vitro (HO induced PC12 cells) via modulating oxidative stress and AChE activity.
薰衣草精油（LO）是从薰衣草中提取的一种芳香液体，传统上用于治疗多种神经系统疾病，最近有报道称LO对阿尔茨海默病（AD）也有效。
评估薰衣草油（LO）对东莨菪碱诱导的小鼠认知缺陷以及对过氧化氢（HO）诱导的PC12细胞毒性的改善作用。并从抗氧化作用和胆碱能系统调节的角度研究相关机制。
用东莨菪碱（1mg/kg，腹腔注射）处理C57BL/6J小鼠诱导认知缺陷，通过莫里斯水迷宫（MWM）和穿梭箱被动回避试验进行评估。然后取出它们的海马进行生化检测（乙酰胆碱酯酶（AChE）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）和丙二醛（MDA））。在体外，PC12细胞暴露于HO 4小时诱导细胞毒性，通过细胞活力（MTT）、乳酸脱氢酶（LDH）水平、一氧化氮（NO）释放、活性氧（ROS）产生和线粒体膜电位（MMP）进行评估。
结果表明，LO（100mg/kg）可改善东莨菪碱诱导小鼠在行为测试中的认知表现。同时，它显著降低模型的AChE活性、MDA水平，并增加SOD和GPX活性。此外，LO（12μg/mL）通过降低LDH、NO释放、细胞内ROS积累和MMP损失，保护PC12细胞免受HO诱导的细胞毒性。
提示LO可通过调节氧化应激和AChE活性，在体内（东莨菪碱处理的小鼠）和体外（HO诱导的PC12细胞）的AD模型中发挥神经保护作用。
