利用石灰钙和灰分进行碱性堆肥消毒的杀菌和杀病毒机制。

Bactericidal and virucidal mechanisms in the alkaline disinfection of compost using calcium lime and ash.

机构信息

Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, North 13, West 8, Kita-ku, Sapporo, Hokkaido 060-8628, Japan.

New Industry Creation Hatchery Center, Tohoku University, Japan.

出版信息

J Environ Manage. 2016 Oct 1;181:721-727. doi: 10.1016/j.jenvman.2016.08.026. Epub 2016 Aug 23.

DOI:10.1016/j.jenvman.2016.08.026

PMID:27562698

Abstract

In the present study, the bactericidal and virucidal mechanisms in the alkaline disinfection of compost with calcium lime and ash were investigated. Two indicator microorganisms, Escherichia coli and MS2 coliphage, were used as surrogates for enteric pathogens. The alkaline-treated compost with calcium oxide (CaO) or ash resulted primarily in damage to the outer membrane and enzyme activities of E. coli. The alkaline treatment of compost also led to the infectivity loss of the coliphage because of the partial capsid damage and RNA exteriorization due to a raised pH, which is proportional to the amount of alkaline agents added. These results indicate that the alkaline treatment of compost using calcium oxide and ash is effective and can contribute to the safe usage of compost from a mixing type dry toilet.

摘要

在本研究中，研究了用石灰和灰分对堆肥进行碱性消毒的杀菌和杀病毒机制。两种指示微生物，大肠杆菌和 MS2 噬菌体，被用作肠道病原体的替代品。用氧化钙（CaO）或灰分处理的碱性堆肥主要导致大肠杆菌的外膜和酶活性受损。由于 pH 值升高导致部分衣壳损伤和 RNA 外显，堆肥的碱性处理也导致噬菌体丧失感染力，而 pH 值与添加的碱性剂的量成正比。这些结果表明，使用氧化钙和灰分对堆肥进行碱性处理是有效的，可以有助于从混合式干式厕所中安全使用堆肥。