Pang Cheng, Liu Ming, Fang Weiwei, Guo Jun, Zhang Zhipeng, Wu Pengjie, Zhang Yaoguang, Wang Jianye
Graduate School of Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.
Cell Physiol Biochem. 2016;39(3):1111-7. doi: 10.1159/000447819. Epub 2016 Aug 26.
Emerging evidence suggested that microRNAs (miRNAs) play a causal role in cancer tumorigenesis. Aberrant expression of miRNA (miR)-139-5p has been observed in various types of cancers. The present study evaluated the relationship between miR-139-5p expression levels and prostate cancer (PCa), to assess the feasibility of using peripheral blood miR-139-5p as a potential non-invasive biomarker for PCa.
Total RNA was extracted from peripheral whole blood samples from 45 PCa patients, 45 benign prostatic hyperplasia (BPH) patients and 50 healthy controls (HC). The expression of miR-139-5p was assessed by reverse transcription quantitative polymerase chain reaction.
MiR-139-5p in peripheral blood was significantly higher in PCa patients than in patients with BPH and HC individuals (P<0.001). Higher miR-139-5p expression was observed to be associated with certain clinicopathological parameters, including PSA>20ng/ml (P<0.05), pathological tumor stage 3/4 (P<0.05) and Gleason score >7 (P<0.01). A receiver operating characteristic (ROC) curve analysis revealed that miR-139-5p distinguished PCa patients from BPH patients [area under the curve (AUC), 0.936; 95% CI, 0.878-0.993; P<0.001].
Peripheral blood miR-139-5p may be utilized as a potential novel non-invasive biomarker for PCa screening.
新出现的证据表明，微小RNA（miRNA）在癌症肿瘤发生中起因果作用。在各种类型的癌症中均观察到miRNA（miR）-139-5p的异常表达。本研究评估了miR-139-5p表达水平与前列腺癌（PCa）之间的关系，以评估将外周血miR-139-5p用作PCa潜在非侵入性生物标志物的可行性。
从45例PCa患者、45例良性前列腺增生（BPH）患者和50例健康对照（HC）的外周全血样本中提取总RNA。通过逆转录定量聚合酶链反应评估miR-139-5p的表达。
PCa患者外周血中的miR-139-5p明显高于BPH患者和HC个体（P<0.001）。观察到较高的miR-139-5p表达与某些临床病理参数相关，包括PSA>20ng/ml（P<0.05）、病理肿瘤分期3/4（P<0.05）和Gleason评分>7（P<0.01）。受试者工作特征（ROC）曲线分析显示，miR-139-5p可区分PCa患者和BPH患者[曲线下面积（AUC），0.936；95%CI，0.878-0.993；P<0.001]。
外周血miR-139-5p可作为PCa筛查的潜在新型非侵入性生物标志物。
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