Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China.
Institute of Orthopaedics and Traumatology of Zhejiang Province, Hangzhou, Zhejiang 310053, China.
Dis Markers. 2019 Nov 15;2019:8759642. doi: 10.1155/2019/8759642. eCollection 2019.
A growing number of studies have suggested that circular RNAs (circRNAs) serve as potential diagnostic biomarkers in many diseases. However, the role of circRNAs in steroid-induced osteonecrosis of the femoral head (SONFH) has not been reported.
Secondary sequencing was performed to profile circRNA expression in peripheral blood samples from three SONFH patients and three healthy individuals. We confirmed our preliminary findings by qRT-PCR. Bioinformatics analysis was conducted to predict their functions.
The result showed 345 dysregulated circRNAs. qRT-PCR of eight selected circRNAs preliminarily confirmed the results, which were consistent with RNA sequencing. Bioinformatics analyses were performed to predict the functions of circRNAs to target the genes of miRNAs and the networks of circRNA-miRNA-mRNA interactions.
This study provides a new and fundamental circRNA profile of SONFH and a theoretical basis for further studies on the functions of circRNAs in SONFH.
越来越多的研究表明，环状 RNA（circRNAs）可作为许多疾病的潜在诊断生物标志物。然而，circRNAs 在激素诱导性股骨头坏死（SONFH）中的作用尚未见报道。
通过对 3 名 SONFH 患者和 3 名健康个体的外周血样本进行二级测序，来描绘 circRNA 的表达情况。我们通过 qRT-PCR 验证了我们的初步发现。进行生物信息学分析以预测它们的功能。
结果显示 345 个差异表达的 circRNAs。对 8 个选定的 circRNAs 进行 qRT-PCR 初步验证，结果与 RNA 测序一致。进行生物信息学分析以预测 circRNAs 靶向 miRNAs 的基因和 circRNA-miRNA-mRNA 相互作用网络的功能。
本研究提供了 SONFH 的全新基本 circRNA 图谱，并为进一步研究 circRNAs 在 SONFH 中的功能提供了理论依据。
