Department of Ultrasound, Qilu Hospital, Shandong University, Jinan 250012, China.
Department of Ultrasound, Qilu Hospital, Shandong University, Jinan 250012, China.
Ultrason Sonochem. 2017 Sep;38:598-603. doi: 10.1016/j.ultsonch.2016.08.022. Epub 2016 Aug 16.
To reveal the effect of tumor cell lines derived from different tissue on sonoporation efficiency under ultrasound microbubble (USMB) treatment, and meanwhile to determine the optimum parameter combination for each tumor cell line. Human breast tumor (MCF-7), ovarian tumor (A2780), liver tumor (Bel7402) and thyroid tumor (ARO) were exposed to ultrasound in the presence of SonoVue MBs. The major parameters for the designed experiments including MB concentration (A1:10%, A2:20%, A3:30%), sound intensity (B1:0.5, B2:1.0, B3:1.5W/cm), irradiation time (C1:30, C2:60, C3:90s). An orthogonal array experimental design based on three levels L9 (3) of the above three parameters was employed to optimize the sonoporation efficiency. MTT experiment was used to calculate cell survival rate. FD500 uptake assay and cytometry were performed to evaluate transference percentage. The optimum parameter combination for each tumor cell line was different (MCF-7: A3B1C1, A2780: A1B3C3, Bel7402: A2B3C2, ARO: A2B3C2). Under their own optimum parameter combination, four kinds of tumor cell line exhibited different optimum sonoporation efficiency (MCF-7: 55.05±5.29%; A2780: 45.62±7.35%; Bel7402: 39.37±4.11%; ARO: 53.37±3.94%). Multiple comparison with LSD-t test showed that the sonoporation efficiency between four kinds of cell line was statistically significant (P<0.05), with the exception of MCF-7 VS. ARO (P=0.487). Tumor cell lines derived from different tissue can impact the sonoporation efficiency, and optimizing the exposure parameters can safely and efficiently increase the cell membrane permeability.
为了揭示不同组织来源的肿瘤细胞系在超声微泡（USMB）治疗下的声孔效应效率，并确定每种肿瘤细胞系的最佳参数组合。将人乳腺癌（MCF-7）、卵巢癌（A2780）、肝癌（Bel7402）和甲状腺癌（ARO）细胞暴露于超声微泡中。实验设计的主要参数包括微泡浓度（A1：10%、A2：20%、A3：30%）、声强（B1：0.5、B2：1.0、B3：1.5W/cm）、辐照时间（C1：30、C2：60、C3：90s）。采用基于上述三个参数的三水平 L9（3）的正交实验设计来优化声孔效应效率。MTT 实验用于计算细胞存活率。FD500 摄取实验和流式细胞术用于评估转染率。每种肿瘤细胞系的最佳参数组合不同（MCF-7：A3B1C1、A2780：A1B3C3、Bel7402：A2B3C2、ARO：A2B3C2）。在其各自的最佳参数组合下，四种肿瘤细胞系表现出不同的最佳声孔效应效率（MCF-7：55.05±5.29%；A2780：45.62±7.35%；Bel7402：39.37±4.11%；ARO：53.37±3.94%）。LSD-t 检验的多重比较显示，四种细胞系的声孔效应效率具有统计学意义（P<0.05），除了 MCF-7 与 ARO 之间（P=0.487）。不同组织来源的肿瘤细胞系会影响声孔效应效率，优化暴露参数可以安全有效地增加细胞膜通透性。
