Bozhenko V K, Kharchenko N V, Vaskevich E F, Kudinova E A, Oorzhak A V, Rozhkova N I, Trotsenko I D
Russian Scientific Center of Roentgenoradiology, Moscow, Russia.
People Friendship University of Russia, Moscow, Russia.
Biomed Khim. 2016 May;62(4):453-7. doi: 10.18097/PBMC20166204453.
Currently, no molecular biological markers do exist for early diagnosis of breast cancer. One of the possible candidates for the marker of early breast cancer is mammaglobin (MGB1) or SCGB2A2 (secretoglobin, family 2A, member 2), characterized by the maximal expression level in early breast cancer. Using the RT-PCR method MGB1 mRNA expression was examined in 57 tumor tissue samples and 57 samples of morphologically non-malignant tissue (MNT) of breast cancer (BC) patients. Specificity and sensitivity of the MGB1 mRNA assay in peripheral blood of BC patients was evaluated by nested PCR. 169 blood samples (from 95 BC patients, 22 from patients with benign breast tumors, 28 from patients with tumors of other localizations, and 24 samples from healthy donors) have been analyzed. MGB1 expression was significantly higher in BC tissue samples compared to MNT (p=0.0019). The maximal expression level was in the samples T1 (p=0.013), stage I BC (p=0.037), GI (p=0.0019). The MGB1 expression positively correlated with expression of estrogen (p = 0,034) and progesterone (p=0.0004) receptors. Sensitivity and specificity of the MGB1 mRNA assay in peripheral blood were 60.6% and 92.3%, respectively. Expression of MGB1 was higher in BC than MNT and it decreased during BC progression. The sensitivity and specificity of the MGB1 mRNA assay may be used as an additional diagnostic method.
目前，尚无用于乳腺癌早期诊断的分子生物学标志物。早期乳腺癌标志物的可能候选物之一是乳腺珠蛋白（MGB1）或SCGB2A2（分泌球蛋白，2A家族，成员2），其特征是在早期乳腺癌中表达水平最高。采用逆转录聚合酶链反应（RT-PCR）方法检测了57例乳腺癌（BC）患者的肿瘤组织样本和57例形态学上非恶性组织（MNT）样本中MGB1 mRNA的表达。通过巢式PCR评估BC患者外周血中MGB1 mRNA检测的特异性和敏感性。分析了169份血样（来自95例BC患者、22例乳腺良性肿瘤患者、28例其他部位肿瘤患者以及24例健康供者）。与MNT相比，BC组织样本中MGB1的表达显著更高（p = 0.0019）。最高表达水平出现在T1期样本（p = 0.013）、I期BC（p = 0.037）、G1期（p = 0.0019）。MGB1表达与雌激素（p = 0.034）和孕激素（p = 0.0004）受体的表达呈正相关。外周血中MGB1 mRNA检测的敏感性和特异性分别为60.6%和92.3%。BC中MGB1的表达高于MNT，且在BC进展过程中降低。MGB1 mRNA检测的敏感性和特异性可作为一种辅助诊断方法。
