炎性牙痛对成年雄性大鼠焦虑的影响。
The Effects of Inflammatory Tooth Pain on Anxiety in Adult Male Rats.
作者信息
Raoof Maryam, Ebrahimnejad Hamed, Abbasnejad Mehdi, Amirkhosravi Ladan, Raoof Ramin, Esmaeili Mahani Saeed, Ramazani Mohsen, Shokouhinejad Noushin, Khoshkhounejad Mehrfam
机构信息
Oral and Dental Diseases Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Department of Oral & Maxillofacial Radiology, Faculty of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Basic Clin Neurosci. 2016 Jul;7(3):259-68. doi: 10.15412/J.BCN.03070311.
INTRODUCTION
This study aimed to examine the effects of induced inflammatory tooth pain on anxiety level in adult male rats.
METHODS
The mandibular incisors of 56 adult male rats were cut off and prefabricated crowns were fixed on the teeth. Formalin and capsaicin were injected intradentally to induce inflammatory tooth pain. Diazepam treated group received diazepam 30 minutes before intradental injection. The anxiety-related behavior was evaluated with elevated plus maze test.
RESULTS
Intradental application of chemical noxious stimuli, capsaicin and formalin, significantly affected nociceptive behaviors (P<0.001). Capsaicin (P<0.001) and formalin (P<0.01) significantly increased the anxiety levels in rats by decrease in the duration of time spent in open arm and increase in the duration of time spent in closed arm. Rats that received capsaicin made fewer open arm entries compared to the control animals (P<0.05). Capsaicin (P<0.001) and formalin (P<0.01) treated rats showed more stretch attend postures compared to the control and sham operated animals. In diazepampretreated rats, capsaicin induced algesic effect was prevented (P<0.001).
CONCLUSION
Inflammatory pulpal pain has anxiogenic effect on rats, whereas diazepam premedication showed both anxiolytic and pain reducing effects.
引言
本研究旨在探讨诱导性炎性牙痛对成年雄性大鼠焦虑水平的影响。
方法
将56只成年雄性大鼠的下颌切牙切断，在牙齿上固定预制牙冠。向牙髓腔内注射福尔马林和辣椒素以诱导炎性牙痛。地西泮治疗组在牙髓腔内注射前30分钟给予地西泮。采用高架十字迷宫试验评估焦虑相关行为。
结果
牙髓腔内应用化学性有害刺激物辣椒素和福尔马林，对伤害性感受行为有显著影响（P<0.001）。辣椒素（P<0.001）和福尔马林（P<0.01）显著增加大鼠的焦虑水平，表现为在开放臂停留时间缩短，在封闭臂停留时间延长。与对照动物相比，接受辣椒素注射的大鼠进入开放臂的次数更少（P<0.05）。与对照和假手术动物相比，接受辣椒素（P<0.001）和福尔马林（P<0.01）治疗的大鼠表现出更多的伸展注意姿势。在地西泮预处理的大鼠中，辣椒素诱导的镇痛作用被阻断（P<0.001）。
结论
炎性牙髓疼痛对大鼠有焦虑诱导作用，而地西泮预处理具有抗焦虑和减轻疼痛的作用。
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