Stachurska Anna, Król Teodora, Trybus Wojciech, Szary Karol, Fabijańska-Mitek Jadwiga
Department of Immunohaematology, Centre of Postgraduate Medical Education, Marymoncka 99/103, 01-813, Warsaw, Poland.
Department of Cell Biology and Electron Microscopy, Institute of Biology, The Jan Kochanowski University, Świętokrzyska 15, 25-406, Kielce, Poland.
Cell Biol Int. 2016 Nov;40(11):1195-1203. doi: 10.1002/cbin.10671. Epub 2016 Sep 13.
Since the erythrophagocytosis of opsonized erythrocytes is investigated mainly by calculating the phagocytic index using subjective light microscopy evaluation, we present methods for the quantitative and qualitative analysis of human cell erythrophagocytosis. Erythrocytes from two storage periods were used. Using Imaris software, we were able to create a three-dimensional model of erythrophagocytosis. The use of microscopy instead of cytometry revealed a significantly higher number of monocytes and erythrocytes that appeared active in phagocytosis. Spatial reconstruction allowed for detailed analysis of the process by precisely locating erythrocytes in phagocytes. Additionally, a technique of sequential image registration using Nis Elements software allowed for observation of the course of phagocytosis over a range of time intervals. This in vitro research may be helpful for understanding the cellular interactions between monocytes and erythrocytes. The cytometric method-being relatively rapid, sensitive, and specific-can serve as an alternative technique to microscopy in the quantitative analysis of erythrophagocytosis. This allows us to avoid counting the erythrocytes nonspecifically attached to monocytes and gives objective results.
由于调理素化红细胞的红细胞吞噬作用主要是通过使用主观光学显微镜评估计算吞噬指数来研究的，我们提出了用于人类细胞红细胞吞噬作用定量和定性分析的方法。使用了两个储存期的红细胞。使用Imaris软件，我们能够创建红细胞吞噬作用的三维模型。使用显微镜而非细胞计数法显示，出现活跃吞噬作用的单核细胞和红细胞数量明显更多。空间重建通过精确确定吞噬细胞中的红细胞位置，实现了对该过程的详细分析。此外，使用Nis Elements软件的序列图像配准技术可以观察一系列时间间隔内的吞噬过程。这项体外研究可能有助于理解单核细胞与红细胞之间的细胞相互作用。细胞计数法相对快速、灵敏且特异，可作为红细胞吞噬作用定量分析中显微镜检查的替代技术。这使我们能够避免对非特异性附着于单核细胞的红细胞进行计数，并给出客观结果。
