Şakalar Ergün
Department of Bioengineering, Faculty of Engineering, Çanakkale Onsekiz Mart University, Canakkale, Turkey.
J Sci Food Agric. 2017 May;97(7):2100-2106. doi: 10.1002/jsfa.8015. Epub 2016 Oct 18.
Ionising radiation induces molecular alterations, such as formation of ions, free radicals, and new stable molecules, and cleavage of the chemical bonds of the molecules present in food. Irradiation-treated meat should be labelled to control the process and to ensure free consumer choice. Therefore, sensitive analytical methods are required to detect the irradiation dose.
Meat samples were exposed to radiation doses of 0, 0.272, 0.497, 1.063, 3.64, 8.82 and 17.42 kGy in an industrial Co gamma cell. Primers were designed to amplify 998, 498 and 250-base pair (bp) regions of the 18S rRNA gene of nuclear DNA from the irradiated samples. A new DNA-based method was developed to quantify the radiation exposed to the unstored meat and the meat stored at -20 °C for 3 and 6 months. The method was able to detect meat samples stored and unstored with dose limits of 1.063 and 3.64 kGy, respectively.
The level of irradiation can be detected using primer pairs that target particularly different-sized sequences for DNA amplification by PCR. This method can be widely used for the analysis of not only meat samples, but also all biological materials containing DNA. © 2016 Society of Chemical Industry.
电离辐射会引起分子变化，例如离子、自由基和新的稳定分子的形成，以及食品中存在的分子化学键的断裂。经过辐照处理的肉类应进行标签标注，以控制该过程并确保消费者能够自由选择。因此，需要灵敏的分析方法来检测辐照剂量。
在工业用钴伽马细胞中，将肉类样品暴露于0、0.272、0.497、1.063、3.64、8.82和17.42千戈瑞的辐射剂量下。设计引物以扩增来自辐照样品的核DNA的18S rRNA基因的998、498和250碱基对（bp）区域。开发了一种基于DNA的新方法，用于量化未储存的肉类以及在-20°C下储存3个月和6个月的肉类所受到的辐射。该方法能够分别检测未储存和储存的肉类样品，剂量限值分别为1.063和3.64千戈瑞。
可以使用引物对来检测辐照水平，这些引物对靶向特定大小不同的序列，用于通过聚合酶链反应（PCR）进行DNA扩增。该方法不仅可广泛用于肉类样品的分析，还可用于所有含有DNA的生物材料的分析。©2016化学工业协会。
