小檗对核心脉冲树突状细胞疫苗免疫调节作用的研究。

Investigation of the Immunomodulatory effect of Berberis vulgaris on core-pulsed dendritic cell vaccine.

作者信息

Ghareeb Doaa A, Elwakeel Eiman H, Khalil Rowaida, Aziz Mina S, El Demellawy Maha A

机构信息

Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.

Center for Aging and Associated Diseases, Helmy Institute for Medical Sciences, Zewail City, Egypt.

出版信息

BMC Complement Altern Med. 2016 Aug 30;16(1):325. doi: 10.1186/s12906-016-1327-2.

DOI:10.1186/s12906-016-1327-2

PMID:27577059

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5004255/

Abstract

BACKGROUND

Virus-induced dendritic cells (DCs) functional deficiency leads to sub-optimal initiation of adaptive immune responses and consequently chronic infection establishment. The present study reports an advanced hepatitis C virus (HCV) therapeutic vaccine model based on In vivo enrichment of DCs with barberry ethanolic crude extract (BCE) then pulsing them with HCV core protein.

METHODS

DCs were enriched by BCE intravenous injection in BALB/c mice. Vaccine efficiency was assessed by flow cytometric analysis of splenocytes of immunized mice, cytokine profiling, cytotoxic T lymphocyte assay, and humoral immune response assessment.

RESULTS

There was no significant difference in surface phenotypic characterization of splenocytes from mice immunized with non-BCE-enriched-core-pulsed DCs (iDcs-core) compared to those from mice injected with RPMI-1640 medium. However, splenocytes from mice immunized with BCE-enriched-core-pulsed DCs showed 197 % increase in CD16+ population, 33 % increase in MHCII(+) population, and 43 % decrease in CD3(+) population. In iDCs-core group, 57.9 % greater anti-core cytotoxic T lymphocyte activity, up-regulation in interferon gamma and interleukin (IL) -12 expression, and down-regulation in IL-4 and IL-10 were recorded. Moreover, sustained specific anti-core antibodies were detected only in sera of the same group.

CONCLUSIONS

results indicate that BCE-enriched-core-transduced DCs may serve as a new model for immunotherapy of HCV chronic infection.

摘要

背景

病毒诱导的树突状细胞（DCs）功能缺陷会导致适应性免疫反应的启动不理想，从而导致慢性感染的建立。本研究报告了一种先进的丙型肝炎病毒（HCV）治疗性疫苗模型，该模型基于体内用小檗乙醇粗提物（BCE）富集DCs，然后用HCV核心蛋白刺激它们。

方法

通过静脉注射BCE在BALB/c小鼠中富集DCs。通过对免疫小鼠脾细胞的流式细胞术分析、细胞因子谱分析、细胞毒性T淋巴细胞测定和体液免疫反应评估来评估疫苗效率。

结果

与注射RPMI-1640培养基的小鼠相比，用未用BCE富集核心刺激的DCs（iDcs-core）免疫的小鼠脾细胞的表面表型特征没有显著差异。然而，用BCE富集核心刺激的DCs免疫的小鼠脾细胞显示CD16+群体增加了197%，MHCII(+)群体增加了33%，CD3(+)群体减少了43%。在iDCs-core组中，抗核心细胞毒性T淋巴细胞活性提高了57.9%，干扰素γ和白细胞介素（IL）-12表达上调，IL-4和IL-10表达下调。此外，仅在同一组的血清中检测到持续的特异性抗核心抗体。