Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
Univ. Estadual Paulista - IBILCE/UNESP, São José do Rio Preto, São Paulo, Brazil.
Bioresour Technol. 2016 Nov;219:773-777. doi: 10.1016/j.biortech.2016.08.075. Epub 2016 Aug 24.
The present work aimed to study the effect of the pretreatment of sugarcane bagasse and straw with microwave irradiation in aqueous and acid glycerol solutions on their chemical composition, fiber structure and the efficiency of subsequent enzymatic hydrolysis. Thermogravimetric analysis showed that the pretreatment acted mainly on the lignin and hemicellulose fractions of the bagasse, whereas, in the straw, lesser structural and chemical changes were observed. The images from transmission electron microscopy (TEM) revealed that treating bagasse and straw with acid glycerol solution loosened the cell walls and there was a breakdown in the pit membrane. The treated material was submitted to hydrolysis for 72h and higher yields of reducing sugars were observed compared to the untreated material (250.9mg/g from straw and 197.4mg/g from bagasse). TEM images after hydrolysis confirmed the possible points of access of the enzymes to the secondary cell wall region of the pretreated biomass.
本工作旨在研究微波辐射预处理甘蔗渣和秸秆在水和酸性甘油溶液中的效果,以及对它们的化学成分、纤维结构和后续酶水解效率的影响。热重分析表明,预处理主要作用于甘蔗渣的木质素和半纤维素部分,而在秸秆中,观察到的结构和化学变化较小。透射电子显微镜(TEM)的图像显示,用酸性甘油溶液处理甘蔗渣和秸秆会使细胞壁变松,并且坑膜破裂。处理后的材料进行 72 小时水解,与未处理的材料相比,还原糖的产率更高(秸秆为 250.9mg/g,甘蔗渣为 197.4mg/g)。水解后的 TEM 图像证实了酶进入预处理生物质次生细胞壁区域的可能切入点。