Chen Hui, Zheng Xiaoyan, Wang Ran, Gao Na, Sheng Ziyang, Fan Dongying, Feng Kaihao, Liao Xianzheng, An Jing
Department of Microbiology and Parasitology, School of Basic Medical Sciences, Chinese Capital Medical University, Beijing 100069, PR China.
Department of Microbiology and Parasitology, School of Basic Medical Sciences, Chinese Capital Medical University, Beijing 100069, PR China; Center of Epilepsy, Beijing Institute for Brain Disorders, Beijing 100069, PR China.
Clin Immunol. 2016 Oct;171:41-49. doi: 10.1016/j.clim.2016.08.021. Epub 2016 Aug 28.
We aimed to use the dengue virus (DV) serotype 2 as a proof of principal for testing the efficacy of a DNA vaccine candidate via in vivo electroporation in mice and rabbits prior to the development of a tetravalent vaccine.
Different dosages of DNA pVAX1-D2ME encoding DV2 prME genes were vaccinated in mice via intramuscular injection or in vivo electroporation, immune responses and protection were determined. In DNA-vaccinated rabbits via electroporation, antibody titer and protein expression were tested.
In mice, 50μg of pVAX1-D2ME via electroporation elicited effective anti-DV2 responses and conferred significant protection against DV2 challenge. Moreover, anti-DV2 IgG and neutralizing antibodies were successfully induced in rabbits immunized with pVAX1-D2ME via electroporation and the expression of the interest protein was observed at local sites.
Enhanced immunogenicity and protective effect conferred by pVAX1-D2ME via electroporation show great promise for the development of a dengue tetravalent DNA vaccine.
在开发四价疫苗之前,我们旨在利用登革病毒2型作为原理验证,通过体内电穿孔在小鼠和兔子中测试候选DNA疫苗的效力。
通过肌肉注射或体内电穿孔将不同剂量编码登革病毒2型prME基因的DNA pVAX1-D2ME接种到小鼠体内,测定免疫反应和保护作用。对通过电穿孔接种DNA疫苗的兔子,检测抗体滴度和蛋白表达。
在小鼠中,通过电穿孔接种50μg pVAX1-D2ME可引发有效的抗登革病毒2型反应,并对登革病毒2型攻击提供显著保护。此外,通过电穿孔用pVAX1-D2ME免疫的兔子成功诱导出抗登革病毒2型IgG和中和抗体,并且在局部部位观察到了目的蛋白的表达。
pVAX1-D2ME通过电穿孔增强的免疫原性和保护作用为登革热四价DNA疫苗的开发展现出巨大前景。