Wan Bingbing, Hang Lisa E, Zhao Xiaolan
a Molecular Biology Program, Memorial Sloan Kettering Cancer Center , New York , NY , USA.
Cell Cycle. 2016 Oct;15(19):2561-2570. doi: 10.1080/15384101.2016.1218102. Epub 2016 Aug 11.
Genome maintenance requires coordinated actions of diverse DNA metabolism processes. Scaffolding proteins, such as those containing multiple BRCT domains, can influence these processes by collaborating with numerous partners. The best-studied examples of multi-BRCT scaffolds are the budding yeast Dpb11 and its homologues in other organisms, which regulate DNA replication, repair, and damage checkpoints. Recent studies have shed light on another group of multi-BRCT scaffolds, including Rtt107 in budding yeast and related proteins in other organisms. These proteins also influence several DNA metabolism pathways, though they use strategies unlike those employed by the Dpb11 family of proteins. Yet, at the same time, these 2 classes of multi-BRCT proteins can collaborate under specific situations. This review summarizes recent advances in our understanding of how these multi-BRCT proteins function in distinct manners and how they collaborate, with a focus on Dpb11 and Rtt107.
基因组维持需要多种DNA代谢过程的协同作用。支架蛋白，如那些含有多个BRCT结构域的蛋白，可以通过与众多伙伴合作来影响这些过程。研究得最透彻的多BRCT支架蛋白的例子是芽殖酵母Dpb11及其在其他生物体中的同源物，它们调节DNA复制、修复和损伤检查点。最近的研究揭示了另一类多BRCT支架蛋白，包括芽殖酵母中的Rtt107和其他生物体中的相关蛋白。这些蛋白也影响几种DNA代谢途径，尽管它们使用的策略与Dpb11家族蛋白不同。然而，与此同时，这两类多BRCT蛋白在特定情况下可以合作。本综述总结了我们在理解这些多BRCT蛋白如何以不同方式发挥作用以及它们如何合作方面的最新进展，重点是Dpb11和Rtt107。
