Thomson S, Wallace A M, Cook B
Department of Bacteriology, Stobhill Hospital, Glasgow, U.K.
Clin Chem. 1989 Aug;35(8):1706-12.
We developed a radioimmunoassay with a gamma-emitting radioligand to measure androstenedione in human serum and in dried blood-spot samples from newborns. Antisera were raised in rabbits against androstenedione linked to bovine serum albumin at positions 3, 6, or 11 on the steroid nucleus. Radioligands were prepared by linking [125I]iodohistamine at positions 3, 6, or 11. Linkages were through either carboxymethyloxime or hemisuccinate bridges. All label and antibody combinations were examined, and the most sensitive and specific combination (antiserum raised against androstenedione-3-carboxymethyloxime-bovine serum albumin with an androstenedione-carboxymethyloxime-[125I]iodohistamine label) was selected for full evaluation. We report the performance of these selected reagents in an immunoassay for androstenedione in both serum and dried blood-spot samples from neonates. We measured concentrations of androstenedione in serum under normal and pathological conditions such as congenital adrenal hyperplasia and polycystic ovarian disease. Diurnal variation in normal men was observed. Androstenedione was measured in blood spots from neonates born at term or prematurely, with respiratory distress syndrome, or with congenital adrenal hyperplasia.
我们开发了一种采用发射γ射线的放射性配体的放射免疫分析法，用于检测人血清及新生儿干血斑样本中的雄烯二酮。用与甾体核上3、6或11位的牛血清白蛋白相连的雄烯二酮免疫家兔制备抗血清。通过将[125I]碘组胺连接在3、6或11位制备放射性配体。连接通过羧甲基肟或半琥珀酸桥进行。对所有标记物与抗体的组合进行了检测，选择了最灵敏且特异的组合（抗雄烯二酮-3-羧甲基肟-牛血清白蛋白抗血清与雄烯二酮-羧甲基肟-[125I]碘组胺标记物）进行全面评估。我们报告了这些选定试剂在检测新生儿血清和干血斑样本中雄烯二酮的免疫分析中的性能。我们测量了正常和病理条件下（如先天性肾上腺增生和多囊卵巢疾病）血清中雄烯二酮的浓度。观察了正常男性的昼夜变化。对足月或早产、患有呼吸窘迫综合征或先天性肾上腺增生的新生儿的血斑中的雄烯二酮进行了检测。
