大鼠肾刷状缘膜囊泡对γ-氨基丁酸的摄取
gamma-Aminobutyric acid uptake by rat kidney brush-border membrane vesicles.
作者信息
Sidhu H S, Wood J D
机构信息
Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.
出版信息
Experientia. 1989 Aug 15;45(8):726-8. doi: 10.1007/BF01974569.
Brush-border membrane vesicles (BBMV) from rat kidney cortex possessed two uptake systems for gamma-aminobutyric acid (GABA), a high affinity system (Km = 10.9 microM) and a low affinity system (Km = 1203 microM). Both uptake systems were inhibited by p-hydroxymercuribenzoic acid and ouabain, and by the action of neuraminidase, whereas the GABA analogs nipecotic acid, beta-alanine, 2,4-diaminobutyric acid and 4,5,6,7-tetrahydroisoxazolo-[4,5 c]-pyridin-3-ol had no effect on the GABA uptake activity. The BBMV uptake systems were clearly different from the GABA transport systems present in brain tissue.
大鼠肾皮质的刷状缘膜囊泡（BBMV）具有两种γ-氨基丁酸（GABA）摄取系统，一种是高亲和力系统（Km = 10.9微摩尔），另一种是低亲和力系统（Km = 1203微摩尔）。两种摄取系统均受到对羟基汞苯甲酸和哇巴因的抑制，也受到神经氨酸酶作用的抑制，而GABA类似物哌啶酸、β-丙氨酸、2,4-二氨基丁酸和4,5,6,7-四氢异恶唑并-[4,5-c]-吡啶-3-醇对GABA摄取活性没有影响。BBMV摄取系统与脑组织中存在的GABA转运系统明显不同。
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