Pinto G P, Lopes K A R, Salles N G, Pacheco-Soares C
Faculdade de Ciências da Saúde, Universidade do Vale do Paraíba (Univap), Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, São Paulo, Brazil.
Instituto de Pesquisa & Desenvolvimento (IP&D) - Laboratório Dinâmica de Compartimentos Celulares, Universidade do Vale do Paraíba (Univap), Av. Shishima Hifumi, 2911, Urbanova, São José dos Campos, São Paulo, Brazil.
J Fluoresc. 2016 Nov;26(6):1923-1926. doi: 10.1007/s10895-016-1914-0. Epub 2016 Sep 5.
The use of lectins can play an important role for tracking modification on cell surface components, since lectins can be easily complexed with radioisotopes, biotin or fluorescein, facilitating the evaluation of carbohydrates distribution in the cell and mitochondrial activity. The aim of this study was to evaluate photodynamic therapy effects on indirect distribution of N-acetyl-glucosamine terminal glycoproteins, in human laryngeal carcinoma HEp-2 cell line surface, using lectin wheat germ agglutinin (WGA) and on mitochondrial activity, for the same cell line, using MitoTracker. The photosensitizer Aluminum Phthalocyanine Tetrasulfonate (AlPcS) was administrated at 10 μM/mL, followed by an incubation period for its accumulation in the tumor cells, which were irradiated with laser diode λ = 685 nm and energy density of 4.5 J/cm. Our results indicated that, after Photodynamic Therapy (PDT), it was observed N-acetyl glucosamine terminal glycoprotein expression and mitochondrial O production, compared to the control group. Based on these results, we suggest that PDT influences the O mitochondrial production and the presence of surface glycoproteins N-acetyl glucosamine terminals.
凝集素的使用对于追踪细胞表面成分的修饰可发挥重要作用，因为凝集素能够轻易地与放射性同位素、生物素或荧光素结合，便于评估细胞中碳水化合物的分布以及线粒体活性。本研究的目的是使用凝集素麦胚凝集素（WGA）评估光动力疗法对人喉癌HEp-2细胞系表面N-乙酰葡糖胺末端糖蛋白间接分布的影响，并使用线粒体追踪染料评估同一细胞系的线粒体活性。以10μM/mL的浓度给予光敏剂四磺基铝酞菁（AlPcS），随后有一个孵育期使其在肿瘤细胞中蓄积，之后用波长λ = 685nm、能量密度为4.5J/cm的激光二极管进行照射。我们的结果表明，与对照组相比，光动力疗法（PDT）后观察到了N-乙酰葡糖胺末端糖蛋白的表达以及线粒体氧的产生。基于这些结果，我们认为PDT会影响线粒体氧的产生以及表面糖蛋白N-乙酰葡糖胺末端的存在。
