Department of Rheumatology, Ajou University School of Medicine, Suwon, Korea.
HLA. 2016 Oct;88(4):187-93. doi: 10.1111/tan.12874. Epub 2016 Sep 6.
MicroRNAs (miRNAs) are short, non-coding RNAs that regulate gene expression at the post-transcriptional level, which can be measured in cells, tissues, and body fluids including plasma. Differences in miRNA expression levels suggest an epigenetic mechanism and changed expression levels are emerging as a novel biomarker for various diseases. We attempted to identify circulating miRNAs associated with susceptibility to systemic lupus erythematosus (SLE) in the Korean population and elucidate their significance for clinical phenotype. An expression profiling analysis using miRNA polymerase chain reaction (PCR) array was conducted with pooled miRNA from 10 patients with SLE and 10 healthy controls (HCs). Nine miRNAs were differentially expressed between the SLE and HC. To verify this, we performed quantitative PCR for various miRNA from SLE patients (n = 70) and HCs (n = 40). The hsa-miR-30e-5p, hsa-miR-92a-3p, and hsa-miR-223-3p were significantly up-regulated in plasma of SLE patients (P = 0.048, P = 0.039, and P = 0.046, respectively). Especially, the hsa-miR-223-3p was significantly associated with oral ulcer (P < 0.001) and lupus anticoagulant (P = 0.031). Thus, plasma hsa-miR-30e-5p, hsa-miR-92a-3p, and hsa-miR-223-3p may be promising novel biomarkers in the diagnosis and clinical manifestation of SLE.
微小 RNA（miRNAs）是短的非编码 RNA，可在转录后水平调节基因表达，可在细胞、组织和体液（包括血浆）中进行测量。miRNA 表达水平的差异提示存在表观遗传机制，并且表达水平的改变正在成为各种疾病的新型生物标志物。我们试图鉴定与韩国人群系统性红斑狼疮（SLE）易感性相关的循环 miRNA，并阐明其对临床表型的意义。使用 miRNA 聚合酶链反应（PCR）阵列对来自 10 名 SLE 患者和 10 名健康对照者（HCs）的混合 miRNA 进行表达谱分析。SLE 和 HCs 之间有 9 个 miRNA 表达存在差异。为了验证这一点，我们对来自 SLE 患者（n = 70）和 HCs（n = 40）的各种 miRNA 进行了定量 PCR。hsa-miR-30e-5p、hsa-miR-92a-3p 和 hsa-miR-223-3p 在 SLE 患者的血浆中明显上调（P = 0.048、P = 0.039 和 P = 0.046）。特别是，hsa-miR-223-3p 与口腔溃疡（P < 0.001）和狼疮抗凝剂（P = 0.031）显著相关。因此，血浆 hsa-miR-30e-5p、hsa-miR-92a-3p 和 hsa-miR-223-3p 可能是 SLE 诊断和临床表现的有前途的新型生物标志物。
