Burgos Rosilene Cristina Rossetto, Červinková Kateřina, van der Laan Tom, Ramautar Rawi, van Wijk Eduard P A, Cifra Michal, Koval Slavik, Berger Ruud, Hankemeier Thomas, van der Greef Jan
Division of Analytical Biosciences, Leiden Academic Center for Drug Research, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; Sino-Dutch Center for Preventive and Personalized Medicine/Center for Photonics of Living Systems, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Institute of Photonics and Electronics, The Czech Academy of Sciences, Chaberská 57, 182 00 Prague, Czech Republic; Faculty of Electrical Engineering, Czech Technical University in Prague, Technická 2, 166 27 Prague, Czech Republic.
J Photochem Photobiol B. 2016 Oct;163:237-45. doi: 10.1016/j.jphotobiol.2016.08.030. Epub 2016 Aug 25.
Ultra-weak photon emission (UPE) is light emitted spontaneously by biological systems without the use of specific luminescent complexes. UPE is emitted in the near-UV/UV-Vis/near-IR spectra during oxidative metabolic reactions; however, the specific pathways involved in UPE remain poorly understood. Here, we used HL-60 cells, a human promyelocytic cell line that is often used to study respiratory burst, as a model system to measure UPE kinetics together with metabolic changes. HL-60 cells were differentiated into neutrophil-like cells by culturing in all-trans-retinoic acid for 7days. We then used a targeted metabolomics approach with capillary electrophoresis-mass spectrometry to profile intracellular metabolites in HL-60 cells and to investigate the biochemical changes based on the measured UPE profile. Our analysis revealed that the levels of specific metabolites, including putrescine, creatine, β-alanine, methionine, hydroxyproline, serine, and S-adenosylmethionine, were significantly altered in HL-60 cells after inducing respiratory burst. A comparison with recorded UPE data revealed that the changes in putrescine, glutathione, sarcosine, creatine, β-alanine, methionine, and hydroxyproline levels were inversely correlated with the change in UPE intensity. These results suggest that these metabolic pathways, particular the methionine pathway, may play a role in the observed changes in UPE in HL-60 cells and therefore demonstrate the potential for using UPE to monitor metabolic changes.
超微弱光子发射（UPE）是生物系统在不使用特定发光复合物的情况下自发发出的光。在氧化代谢反应过程中，UPE在近紫外/紫外-可见/近红外光谱范围内发射；然而，UPE所涉及的具体途径仍知之甚少。在这里，我们使用HL-60细胞（一种常用于研究呼吸爆发的人早幼粒细胞系）作为模型系统，来测量UPE动力学以及代谢变化。通过在全反式维甲酸中培养7天，将HL-60细胞分化为嗜中性粒细胞样细胞。然后，我们采用毛细管电泳-质谱靶向代谢组学方法，对HL-60细胞内的代谢物进行分析，并根据测得的UPE图谱研究其生化变化。我们的分析表明，在诱导呼吸爆发后，HL-60细胞中包括腐胺、肌酸、β-丙氨酸、蛋氨酸、羟脯氨酸、丝氨酸和S-腺苷甲硫氨酸在内的特定代谢物水平发生了显著变化。与记录的UPE数据进行比较发现，腐胺、谷胱甘肽、肌氨酸、肌酸、β-丙氨酸、蛋氨酸和羟脯氨酸水平的变化与UPE强度的变化呈负相关。这些结果表明，这些代谢途径，特别是蛋氨酸途径，可能在HL-60细胞中观察到的UPE变化中起作用，因此证明了利用UPE监测代谢变化的潜力。
