Partridge C A, Weinstein B I, Southren A L, Gerritsen M E
Department of Physiology, New York Medical College, Valhalla 10595.
Invest Ophthalmol Vis Sci. 1989 Aug;30(8):1843-7.
Previous studies have demonstrated the presence of high-affinity, glucocorticoid-specific receptors in explants of human outflow tissue and in cultured trabecular meshwork. Glucocorticoid-induced responses of scleral fibroblasts and trabecular meshwork cells were evaluated in this study. Incubation of human trabecular meshwork (HTM) and scleral fibroblast (HS) cells with 10(-7) M dexamethasone (DEX) results in a 60% inhibition of prostaglandin production. The effects of glucocorticoid treatment on cellular and secreted proteins using [35S] methionine incorporation were evaluated. Treatment of HTM cells cultured from two normal individuals with DEX induced the expression of [35S] methionine-labelled cellular proteins of 35, 65 and 70 kD, and secreted proteins of 40, 90 and 100 kD. Under the same experimental conditions, a 70 kD molecular weight cellular protein was induced in the HS cells. There were no apparent DEX-induced alterations in HS-secreted proteins. Since a functional common response to glucocorticoid treatment in both HS and HTM cells was inhibition of prostaglandin production, the dexamethasone-induced expression of the 70 kD protein in these cells may be related to this effect. Further studies are required to elucidate specific roles of the steroid-induced proteins in the effects of glucocorticoids on HTM and HS cells.
以往的研究已证实在人房水流出组织外植体和培养的小梁网中存在高亲和力、糖皮质激素特异性受体。本研究评估了糖皮质激素对巩膜成纤维细胞和小梁网细胞的诱导反应。用10(-7)M地塞米松（DEX）孵育人小梁网（HTM）和巩膜成纤维细胞（HS），可使前列腺素生成受到60%的抑制。利用[35S]甲硫氨酸掺入法评估了糖皮质激素处理对细胞蛋白和分泌蛋白的影响。用DEX处理来自两名正常个体培养的HTM细胞，诱导了35、65和70kD的[35S]甲硫氨酸标记的细胞蛋白以及40、90和100kD的分泌蛋白的表达。在相同实验条件下，HS细胞中诱导出一种70kD分子量的细胞蛋白。HS分泌蛋白未出现明显的DEX诱导改变。由于HS和HTM细胞对糖皮质激素处理的共同功能反应是抑制前列腺素生成，这些细胞中地塞米松诱导的70kD蛋白表达可能与此效应有关。需要进一步研究以阐明类固醇诱导蛋白在糖皮质激素对HTM和HS细胞作用中的具体作用。
