Wang Zhiyong, Tan Yuanqing, Xu Ding, Wang Gejiao, Yuan Jihong, Zheng Shixue
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
Xianning Vocational Technical College, Xianning, 437100, PR China.
Int J Syst Evol Microbiol. 2016 Dec;66(12):5112-5117. doi: 10.1099/ijsem.0.001480. Epub 2016 Sep 5.
A Gram-stain-negative, light pink, non-motile, rod-shaped, aerobic bacterium, designated strain XNV015T, was isolated from soil of a vanadium mine. Phylogenetic analyses based on 16S rRNA gene sequences indicated that it belongs to the genus Pedobacter and was closely related to Pedobacter suwonensis DSM 18130T (96.93 % sequence similarity), Pedobacter alluvionis NWER-II11T (96.66 %), Pedobacter terrae DS-57T (96.54 %), Pedobacter kyungheensis KACC 16221T (96.54 %) and Pedobacter soli KACC 14939T (96.47 %). This strain clearly differed from the closely related species in terms of acid production from rhamnose and ethanol. Menaquinone-7 was the predominant respiratory quinone. The predominant fatty acids included iso-C15 : 0, C16 : 1ω5c, summed feature 3, iso-C17 : 0 3-OH and C17 : 0 2-OH. The major polar lipids were phosphatidylethanolamine, glycolipids, lipids and aminolipids. The genomic DNA G+C content was 43.8 mol%. The genotypic analysis, biochemical properties, and phenotypic and chemotaxonomic characteristics indicate that strain XNV015T represents a novel species of the genus Pedobacter, for which the name Pedobacter vanadiisoli sp. nov. is proposed. The type strain is XNV015T (=CCTCC AB 2015319T=KCTC 42866T).
从钒矿土壤中分离出一株革兰氏染色阴性、浅粉色、无运动性、杆状的需氧细菌，命名为菌株XNV015T。基于16S rRNA基因序列的系统发育分析表明，它属于Pedobacter属，与水原Pedobacter菌DSM 18130T（序列相似性96.93%）、冲积Pedobacter菌NWER-II11T（96.66%）、土壤Pedobacter菌DS-57T（96.54%）、庆熙Pedobacter菌KACC 16221T（96.54%）和土壤Pedobacter菌KACC 14939T（96.47%）密切相关。该菌株在鼠李糖和乙醇产酸方面与密切相关物种明显不同。甲基萘醌-7是主要的呼吸醌。主要脂肪酸包括异-C15 : 0、C16 : 1ω5c、总和特征3、异-C17 : 0 3-OH和C17 : 0 2-OH。主要极性脂类为磷脂酰乙醇胺、糖脂、脂类和氨基脂类。基因组DNA G+C含量为43.8 mol%。基因型分析、生化特性以及表型和化学分类特征表明，菌株XNV015T代表Pedobacter属的一个新物种，为此提出名为钒矿土壤Pedobacter菌（Pedobacter vanadiisoli sp. nov.）的新名称。模式菌株为XNV015T（=CCTCC AB 2015319T=KCTC 42866T）。
