Zhu X X, Kozarsky K, Strahler J R, Eckerskorn C, Lottspeich F, Melhem R, Lowe J, Fox D A, Hanash S M, Atweh G F
Veterans Administration Medical Center, University of Michigan, Ann Arbor 48109.
J Biol Chem. 1989 Aug 25;264(24):14556-60.
We have recently described an 18-kilodalton polypeptide (p18) that is present in much greater abundance in acute leukemic blast cells (myeloid and lymphoid) than in resting or proliferating nonleukemic lymphoid cells or chronic lymphoid and myeloid leukemic cells. In this report we describe the cloning of two different sized full-length cDNAs that code for p18. The two cDNAs differ in their 3'-noncoding regions as a result of alternative polyadenylation. Analysis of the complete nucleotide sequence and the corresponding amino acid sequence did not reveal significant homology to any previously described sequences. We show evidence that this gene is highly conserved in several animal species and low stringency hybridization studies suggest that the p18 gene may be a member of a family of partially homologous genes in the human genome.
我们最近描述了一种18千道尔顿的多肽（p18），它在急性白血病原始细胞（髓系和淋系）中的丰度比静止或增殖的非白血病淋巴细胞以及慢性淋巴细胞和髓系白血病细胞中高得多。在本报告中，我们描述了编码p18的两种不同大小全长cDNA的克隆。由于可变聚腺苷酸化，这两种cDNA的3'非编码区有所不同。对完整核苷酸序列和相应氨基酸序列的分析未发现与任何先前描述的序列有明显同源性。我们有证据表明该基因在几种动物物种中高度保守，低严谨度杂交研究表明p18基因可能是人类基因组中部分同源基因家族的成员。
