Thomas Funmilola C, Waterston Mary, Hastie Peter, Haining Hayley, Eckersall P David
Department of Veterinary Physiology and Pharmacology,College of Veterinary Medicine,Federal University of Agriculture,Abeokuta,Nigeria.
Institute of Infection,Immunity and Inflammation,College of Veterinary, Medical and Life Sciences,University of Glasgow,Glasgow,UK.
J Dairy Res. 2016 Aug;83(3):352-9. doi: 10.1017/S0022029916000297.
The periparturient period is one of the most critical periods in the productive life of a dairy cow, and is the period when dairy cows are most susceptible to developing new intramammary infections (IMI) leading to mastitis. Acute phase proteins (APP) such as haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) have been detected in milk during mastitis but their presence in colostrum and milk in the immediate postpartum period has had limited investigation. The hypothesis was tested that APP are a constituent of colostrum and milk during this period. Enzyme linked immunosorbent assays (ELISAs) were used to determine each APP's concentration in colostrum and milk collected daily from the first to tenth day following calving in 22 Holstein-Friesian dairy cows. Haptoglobin was assessed in individual quarters and composite milk samples while M-SAA3 and CRP concentration were determined in composite milk samples. Change in Hp in relation to the high abundance proteins during the transition from colostrum to milk were evaluated by 1 and 2 dimension electrophoresis and western blot. In 80% of the cows all APPs were detected in colostrum on the first day following parturition at moderately high levels but gradually decreased to minimal values in the milk by the 6th day after calving. The remaining cows (20%) showed different patterns in the daily milk APP concentrations and when an elevated level is detected could reflect the presence of IMI. Demonstration that APP are present in colostrum and milk following parturition but fall to low levels within 4 days means that elevated APP after this time could be biomarkers of post parturient mastitis allowing early intervention to reduce disease on dairy farms.
围产期是奶牛生产周期中最关键的时期之一，也是奶牛最易发生导致乳腺炎的新的乳房内感染（IMI）的时期。在乳腺炎期间，已在牛奶中检测到急性期蛋白（APP），如触珠蛋白（Hp）、乳腺相关血清淀粉样蛋白A3（M-SAA3）和C反应蛋白（CRP），但它们在产后即刻初乳和牛奶中的存在情况研究有限。本研究检验了这一假说：在此期间APP是初乳和牛奶的组成成分。采用酶联免疫吸附测定（ELISA）法测定了22头荷斯坦-弗里生奶牛产后第1天至第10天每天采集的初乳和牛奶中各APP的浓度。对单个乳腺区和混合乳样中的触珠蛋白进行了评估，而M-SAA3和CRP浓度则在混合乳样中测定。通过一维和二维电泳及蛋白质印迹法评估了从初乳到牛奶转变过程中Hp相对于高丰度蛋白的变化。在80%的奶牛中，所有APP在产后第1天的初乳中均被检测到，且水平适中偏高，但到产后第6天时在牛奶中逐渐降至最低值。其余20%的奶牛在每日牛奶APP浓度上呈现不同模式，检测到水平升高时可能反映存在IMI。产后初乳和牛奶中存在APP但在4天内降至低水平，这表明在此之后APP水平升高可能是产后乳腺炎的生物标志物，有助于早期干预以减少奶牛场的疾病发生。
