Ruminant and Swine Clinic, Free University of Berlin, Berlin, Germany.
Institute for Veterinary Epidemiology and Biostatistics, Free University of Berlin, Berlin, Germany.
J Dairy Res. 2021 Feb;88(1):33-37. doi: 10.1017/S0022029921000078. Epub 2021 Feb 17.
This research communication describes (1) the comparison of acute-phase protein (APP) concentrations in transition dairy cows on different farms using both pooled and individual blood samples, and (2) the association among different APP and clinical health parameters. The first hypothesis was that early postpartum dairy cows from different farms differ in the level of inflammation, which might be determined using APP assays in both pooled and individual blood samples. The second hypothesis was that the APP haptoglobin (Hp) might be the most sensitive parameter to detect cows at risk of excessive postpartum inflammation concomitant with systemic disease. Serum concentrations of Hp, serum amyloid-A (SAA), total protein (TP), albumin (Alb), coeruloplasmin (Cp) and C-reactive protein (CRP) in 100 fresh lactating cows (within 0-8 d postpartum) from 10 farms were compared and associated to clinical health parameters (rectal body temperature, vaginal discharge (Metricheck™ score), rumen fill, vulvovaginal laceration) using both pooled and individual blood samples. Mean serum concentrations of Hp, SAA and TP revealed significant differences among farms. Pooled serum samples of farms showed high correlations with the mean of individual samples. Only Hp was significantly positively correlated to both body temperature and Metricheck™ score. In conclusion, Hp differentiates dairy farms regarding the inflammatory state of transition cows using individual and pooled serum samples within the first week postpartum. It also mirrors the individual degree of inflammation, thus proving to be a diagnostic parameter of high interest during the periparturient period.
本研究通讯描述了（1）使用混合和个体血液样本比较不同农场处于过渡阶段奶牛的急性期蛋白（APP）浓度，以及（2）不同 APP 与临床健康参数之间的关系。第一个假设是，来自不同农场的产后早期奶牛在炎症水平上存在差异，这可以使用混合和个体血液样本中的 APP 检测来确定。第二个假设是，触珠蛋白（Hp）可能是检测产后过度炎症伴全身疾病风险奶牛的最敏感参数。在 10 个农场的 100 头新鲜泌乳奶牛（产后 0-8 天内）中比较了 Hp、血清淀粉样蛋白 A（SAA）、总蛋白（TP）、白蛋白（Alb）、铜蓝蛋白（Cp）和 C-反应蛋白（CRP）的血清浓度，并使用混合和个体血液样本将其与临床健康参数（直肠体温、阴道分泌物（Metricheck™评分）、瘤胃充盈度、外阴阴道撕裂）相关联。Hp、SAA 和 TP 的平均血清浓度在农场之间存在显著差异。农场的混合血清样本与个体样本的平均值高度相关。只有 Hp 与体温和 Metricheck™评分均呈显著正相关。总之，Hp 使用产后第一周内的个体和混合血清样本，区分了过渡奶牛的炎症状态。它还反映了个体炎症程度，因此在围产期期间是一个非常有价值的诊断参数。
