Smith R E, MacQuarrie R A, Jope R S
School of Pharmacy, University of Missouri-Kansas City 64110.
J Chromatogr Sci. 1989 Aug;27(8):491-5. doi: 10.1093/chromsci/27.8.491.
Anion chromatography methods have been developed to determine the concentrations of inositol phosphates and other water-soluble metabolites in rat brain. The cerebral cortex, hippocampus, and striatum were analyzed by chemically suppressed conductivity detection. Animals were sacrificed by directed microwave irradiation for more accurate estimations of in vivo concentrations. The effects of different sample preparation methods are compared. Trichloroacetic acid, formic acid, and perchloric acid extraction result in lower recoveries of phosphocreatine and other anions than does homogenization with water followed by chloroform-methanol extraction. The effects of postdecapitative ischemia on metabolite concentrations are determined. These methods will be used to study the effects of pharmacological agents on inositol phosphates and should be broadly applicable to the analysis of a variety of biological systems.
已开发出阴离子色谱法来测定大鼠脑中肌醇磷酸酯和其他水溶性代谢物的浓度。通过化学抑制电导检测法对大脑皮层、海马体和纹状体进行分析。为了更准确地估计体内浓度，采用定向微波辐射法处死动物。比较了不同样品制备方法的效果。与用水匀浆后再用氯仿 - 甲醇萃取相比，三氯乙酸、甲酸和高氯酸萃取导致磷酸肌酸和其他阴离子的回收率较低。确定了断头后缺血对代谢物浓度的影响。这些方法将用于研究药理试剂对肌醇磷酸酯的影响，并且应广泛适用于各种生物系统的分析。
