Kitahama K, Sallanon M, Lin J S, Maeda T, Jouvet M
Départment de Médecine Expérimentale, INSERM U52, CNRS UA1195, Faculté de Médecine, Lyon, France.
J Comp Neurol. 1989 Jul 8;285(2):218-30. doi: 10.1002/cne.902850205.
The present study, using a diaminobenzidine (DAB)-coupled peroxidation method, examined the distribution and morphological characteristics of neuronal structures containing type B monoamine oxidase (MAO-B) in the cat hypothalamus. Large and intensely stained, distinctive MAO-B-positive cells, multipolar and with long dendritic arbors, were principally distributed in the ventral hypothalamus extending from A7 to A12.5 of the Horsley-Clarke plane. These cells were located caudally in the ventral surface of the brain including the tuberomamillary nucleus (TM) and the region surrounding the mamillary nuclei. Rostrally, they were aggregated in the area surrounding the fornix, particularly in the lateral perifornical region, and dispersed in the anterior mamillary nucleus, lateral hypothalamic area (HLA), and the medial tip of the entopeduncular nucleus. The most rostral positive cell group was identified in a narrow space between the optic tract and the entopeduncular nucleus at the A12.5 level. In addition to these large cells, the present study disclosed the presence of "small" to "very small" MAO-B-positive cells in the area surrounding the mamillary recess and the lateral part of the caudal arcuate nucleus. Distinct MAO-B-stained fibers were identified in all regions of the hypothalamus. A large number of thick labeled fibers were observed in the ventral hypothalamus including the TM and premamillary nucleus and posterior and lateral hypothalamic areas. A dense network of MAO-B-positive terminal-like fibers was observed in the dorsomedial nucleus where very small labeled cells were scattered. Many intensely stained thick and straight fibers were seen running ventrolaterally in the anterior part of the HLA and in the narrow space between the entopeduncular nucleus and optic tract. In the area of the tuber cinereum and the ventral part of the HLA, there were many positive fibers cut transversely, possibly projecting to the more anterior parts of the brain such as the diagonal band of Broca or septal nuclei.
本研究采用二氨基联苯胺（DAB）偶联过氧化法，检测了猫下丘脑内含有B型单胺氧化酶（MAO - B）的神经元结构的分布及形态特征。大的、染色深且独特的MAO - B阳性细胞，多极且有长树突分支，主要分布在从霍斯利 - 克拉克平面的A7延伸至A12.5的下丘脑腹侧。这些细胞位于脑腹侧表面的尾侧，包括乳头体结节核（TM）和乳头体核周围区域。在头侧，它们聚集在穹窿周围区域，特别是外侧穹窿周区，并分散在前乳头体核、下丘脑外侧区（HLA）和内囊核内侧尖端。最头侧的阳性细胞群位于A12.5水平视束和内囊核之间的狭窄空间内。除了这些大细胞外，本研究还揭示在乳头体隐窝周围区域和尾侧弓状核外侧部分存在“小”到“非常小”的MAO - B阳性细胞。在下丘脑的所有区域均鉴定出明显的MAO - B染色纤维。在包括TM和乳头体前核以及下丘脑后部和外侧区的下丘脑腹侧观察到大量粗大的标记纤维。在背内侧核中观察到MAO - B阳性终末样纤维的密集网络，其中散布着非常小标有细胞。在HLA前部以及内囊核和视束之间的狭窄空间内可见许多染色深的粗而直的纤维向腹外侧走行。在灰结节区域和HLA腹侧部分，有许多横向切断的阳性纤维，可能投射到大脑更靠前的部分，如布洛卡斜带或隔核。
