Matsui Katsuhiro, Hisano Tomomi, Yasui Yasuo, Mori Masashi, Walker Amanda R, Morishita Toshikazu, Katsu Kenjiro
NARO Kyushu Okinawa Agricultural Research Center, 2421 Suya, Koshi, Kumamoto 861-1192, Japan.
NARO Kyushu Okinawa Agricultural Research Center, 2421 Suya, Koshi, Kumamoto 861-1192, Japan.
J Plant Physiol. 2016 Oct 20;205:41-47. doi: 10.1016/j.jplph.2016.08.010. Epub 2016 Aug 28.
Proanthocyanidins (PAs) are a major group of flavonoids synthesized via the phenylpropanoid biosynthesis pathway, however the pathway has not been fully characterized in buckwheat. Anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) are involved in the last steps of PA biosynthesis. To isolate the genes for these enzymes from buckwheat we performed PCR using degenerate primers and obtained cDNAs of ANR and LAR, which we designated FeANR and FeLAR1. A search for homologs in a buckwheat genome database with both sequences returned two more LAR sequences, designated FeLAR2 and FeLAR3. Linkage analysis with an F segregating population indicated that the three LAR loci were not genetically linked. We detected high levels of PAs in roots and cotyledons of buckwheat seedlings and in buds and flowers of mature plants. FeANR and FeLAR1-3 were expressed in most organs but had different expression patterns. Our findings would be useful for breeding and further analysis of PA synthesis and its regulation in buckwheat.
原花青素（PAs）是通过苯丙烷生物合成途径合成的一类主要黄酮类化合物，然而该途径在荞麦中尚未得到充分表征。花青素还原酶（ANR）和无色花青素还原酶（LAR）参与了PA生物合成的最后步骤。为了从荞麦中分离出这些酶的基因，我们使用简并引物进行了PCR，并获得了ANR和LAR的cDNA，我们将其命名为FeANR和FeLAR1。用这两个序列在荞麦基因组数据库中搜索同源物，又得到了另外两个LAR序列，命名为FeLAR2和FeLAR3。利用F分离群体进行连锁分析表明，三个LAR位点没有遗传连锁关系。我们在荞麦幼苗的根和子叶以及成熟植株的芽和花中检测到高水平的PAs。FeANR和FeLAR1-3在大多数器官中表达，但具有不同的表达模式。我们的研究结果将有助于荞麦PA合成及其调控的育种和进一步分析。
