Llavador A, Sola-Pikabea J, Saavedra G, Javidi B, Martínez-Corral M
Opt Express. 2016 Sep 5;24(18):20792-8. doi: 10.1364/OE.24.020792.
Integral microscopes (IMic) have been recently developed in order to capture the spatial and the angular information of 3D microscopic samples with a single exposure. Computational post-processing of this information permits to carry out a 3D reconstruction of the sample. By applying conventional algorithms, both depth and also view reconstructions are possible. However, the main drawback of IMic is that the resolution of the reconstructed images is low and axially heterogeneous. In this paper, we propose a new configuration of the IMic by placing the lens array not at the image plane, but at the pupil (or Fourier) plane of the microscope objective. With this novel system, the spatial resolution is increased by factor 1.4, and the depth of field is substantially enlarged. Our experiments show the feasibility of the proposed method.
积分显微镜（IMic）是最近开发出来的，用于在单次曝光下捕捉三维微观样本的空间和角度信息。对这些信息进行计算后处理能够实现样本的三维重建。通过应用传统算法，可以进行深度重建和视图重建。然而，积分显微镜的主要缺点是重建图像的分辨率较低且轴向不均匀。在本文中，我们提出了一种积分显微镜的新配置，即将透镜阵列放置在显微镜物镜的光瞳（或傅里叶）平面，而不是像平面。使用这种新颖的系统，空间分辨率提高了1.4倍，景深也大幅扩大。我们的实验证明了该方法的可行性。
