Ugawa Masashi, Ota Sadao
Research Center for Advanced Science and Technology, University of Tokyo, Tokyo 153-8904, Japan.
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94143, USA.
Cells. 2024 Dec 16;13(24):2073. doi: 10.3390/cells13242073.
Imaging flow cytometry is a technology that performs microscopy image analysis of cells within flow cytometry and allows high-throughput, high-content cell analysis based on their intracellular molecular distribution and/or cellular morphology. While the technology has been available for a couple of decades, it has recently gained significant attention as technical limitations for higher throughput, sorting capability, and additional imaging dimensions have been overcome with various approaches. These evolutions have enabled imaging flow cytometry to offer a variety of solutions for life science and medicine that are not possible with conventional flow cytometry or microscopy-based screening. It is anticipated that the extent of applications will expand in the upcoming years as the technology becomes more accessible through dissemination. In this review, we will cover the technical advances that have led to this new generation of imaging flow cytometry, focusing on the advantages and limitations of each technique.
成像流式细胞术是一种在流式细胞仪内对细胞进行显微镜图像分析的技术，它能够基于细胞内分子分布和/或细胞形态进行高通量、高内涵的细胞分析。尽管这项技术已经问世几十年了，但随着通过各种方法克服了高通量、分选能力和额外成像维度方面的技术限制，它最近受到了广泛关注。这些进展使成像流式细胞术能够为生命科学和医学提供各种传统流式细胞术或基于显微镜的筛选无法实现的解决方案。预计随着该技术通过传播变得更加容易获得，其应用范围将在未来几年得到扩展。在这篇综述中，我们将介绍促成新一代成像流式细胞术的技术进展，重点关注每种技术的优缺点。
