Hayunga E G, Sumner M P, Letonja T
U.S. Department of Agriculture, Food Safety and Inspection Service, Beltsville, Maryland 20705.
J Parasitol. 1989 Aug;75(4):638-42.
Strobilocerci of Taenia taeniaeformis were obtained from laboratory rats 90 days after experimental infection. Cyst fluid, whole parasite homogenate, and rat serum each were fractionated by SDS-PAGE, immobilized on nitrocellulose by western blot, and probed with conjugated goat anti-rat IgG. Reactive bands with relative mobilities corresponding to rat IgG were found in all 3 samples. Additional bands in cyst fluid and parasite homogenate may represent enzymatic degradation of IgG. The pattern of reactive bands in the homogenate discounts the nonspecific adsorption of host molecules onto the tegument and suggests selective incorporation of serum proteins. The presence of an IgG-like molecule of atypical molecular weight is consistent with either molecular mimicry or enzymatic cleavage of IgG bound to the tegument. The relevance of serum protein utilization by the parasite to evasion of the host immune response is discussed.
实验感染90天后，从实验室大鼠体内获取了泡状带绦虫的链尾蚴。将囊液、完整寄生虫匀浆和大鼠血清分别进行SDS - PAGE分离，通过蛋白质印迹法固定在硝酸纤维素膜上，并用偶联的山羊抗大鼠IgG进行检测。在所有3个样品中均发现了相对迁移率与大鼠IgG相对应的反应条带。囊液和寄生虫匀浆中的其他条带可能代表IgG的酶解产物。匀浆中反应条带的模式排除了宿主分子非特异性吸附到皮层上的可能性，并提示血清蛋白的选择性掺入。非典型分子量的IgG样分子的存在与分子模拟或与皮层结合的IgG的酶解作用一致。文中讨论了寄生虫利用血清蛋白与逃避宿主免疫反应的相关性。
