Li Shanshan, Wang Shan, Yan Wei
Department of Environmental Science & Engineering, Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China.
Int J Environ Res Public Health. 2016 Sep 6;13(9):883. doi: 10.3390/ijerph13090883.
Co-metabolic bioremediation is supposed to be an impressive and promising approach in the elimination technology of methyl tert-butyl ether (MTBE), which was found to be a common pollutant worldwide in the ground or underground water in recent years. In this paper, bacterial strain DZ13 (which can co-metabolically degrade MTBE) was isolated and named as Pseudomonas sp. DZ13 based on the result of 16S rRNA gene sequencing analysis. Strain DZ13 could grow on n-alkanes (C₅-C₈), accompanied with the co-metabolic degradation of MTBE. Diverse n-alkanes with different carbon number showed a significant influence on the degradation rate of MTBE and accumulation of tert-butyl alcohol (TBA). When Pseudomonas sp. DZ13 co-metabolically degraded MTBE with n-pentane as the growth substrate, a higher MTBE-degrading rate (Vmax = 38.1 nmol/min/mgprotein, Ks = 6.8 mmol/L) and lower TBA-accumulation was observed. In the continuous degradation experiment, the removal efficiency of MTBE by Pseudomonas sp. Strain DZ13 did not show an obvious decrease after five times of continuous addition.
共代谢生物修复被认为是甲基叔丁基醚（MTBE）去除技术中一种令人印象深刻且很有前景的方法，MTBE近年来在全球范围内的地表水或地下水中被发现是一种常见污染物。在本文中，分离出了能共代谢降解MTBE的细菌菌株DZ13，并根据16S rRNA基因测序分析结果将其命名为假单胞菌属DZ13。菌株DZ13能够在正构烷烃（C₅ - C₈）上生长，并伴随着MTBE的共代谢降解。不同碳数的正构烷烃对MTBE的降解速率和叔丁醇（TBA）的积累有显著影响。当假单胞菌属DZ13以正戊烷作为生长底物共代谢降解MTBE时，观察到较高的MTBE降解速率（Vmax = 38.1 nmol/min/mg蛋白，Ks = 6.8 mmol/L）和较低的TBA积累。在连续降解实验中，假单胞菌属菌株DZ13对MTBE的去除效率在连续添加五次后并未明显降低。
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