棉酚诱导的大鼠附睾精子微环境变化

Gossypol-induced modifications in the microenvironment of rat epididymal spermatozoa.

作者信息

Soufir J C, Radigue C, Dantec M C, Garnier D, Jegou B

机构信息

Laboratoire de Biologie Cellulaire, Faculté de Médecine de Paris-Sud, Kremlin Bicêtre, France.

出版信息

J Reprod Fertil. 1989 Jul;86(2):427-34. doi: 10.1530/jrf.0.0860427.

DOI:10.1530/jrf.0.0860427

PMID:2760873

Abstract

Gossypol acetic acid (20, 25 or 30 mg/kg/day orally for 5 weeks) decreased epididymal weight in adult Sprague-Dawley rats but the epididymal concentrations of proteins, lactate dehydrogenase and acid phosphatase were unchanged. The concentrations of carnitine, inositol and potassium in epididymal fluid were decreased in a dose-related manner. These modifications were not due to disturbances of Leydig and Sertoli cell functions which were normal. We suggest that the reduction in epididymal secretion results from a decrease in the number of spermatozoa rather than from a direct action of gossypol on the epididymal epithelium.

摘要

醋酸棉酚（以20、25或30毫克/千克/天的剂量口服，持续5周）可使成年斯普拉格-道利大鼠的附睾重量减轻，但附睾中的蛋白质、乳酸脱氢酶和酸性磷酸酶浓度未发生变化。附睾液中肉碱、肌醇和钾的浓度呈剂量依赖性降低。这些改变并非由于正常的睾丸间质细胞和支持细胞功能受到干扰所致。我们认为，附睾分泌物的减少是由于精子数量减少，而非棉酚对附睾上皮的直接作用。