Kühlbrandt W, Downing K H
European Molecular Biology Laboratory, Heidelberg, West Germany.
J Mol Biol. 1989 Jun 20;207(4):823-8. doi: 10.1016/0022-2836(89)90247-7.
The structure of the light-harvesting chlorophyll a/b-protein complex has been determined at 3.7 A resolution in projection by electron diffraction, electron microscopy and image analysis. Diffraction patterns and high-resolution spotscan images of two-dimensional crystals stabilized with tannin were recorded at low temperature. Phases of structure factors were obtained directly by image processing, after correction of the images for lattice distortions, defocus and beam tilt. Amplitudes were measured by electron diffraction. The projection map shows the detailed structure of the trimeric complex, suggesting the positions of two domains of potential structural and functional homology, of one membrane-spanning alpha-helix approximately perpendicular to the membrane plane and of several tightly bound lipid molecules.
通过电子衍射、电子显微镜和图像分析，已在3.7埃分辨率下以投影方式确定了捕光叶绿素a/b蛋白复合物的结构。在低温下记录了用单宁稳定的二维晶体的衍射图案和高分辨率点扫描图像。在对图像进行晶格畸变、散焦和束倾斜校正后，通过图像处理直接获得结构因子的相位。通过电子衍射测量振幅。投影图显示了三聚体复合物的详细结构，表明了两个具有潜在结构和功能同源性的结构域、一个大致垂直于膜平面的跨膜α螺旋以及几个紧密结合的脂质分子的位置。
