Guillén-Muñoz J M, Meza-Herrera C A, Santos-Jimenez Z, Rivas-Muñoz R, Luna-Orozco J R, Mellado M, Véliz-Deras F G
Universidad Autónoma Agraria Antonio Narro-Unidad Laguna, Torreón, Coahuila, 27054, Mexico.
Universidad Autónoma Chapingo, Unidad Regional Universitaria de Zonas Áridas, Bermejillo, Durango, 35230, Mexico.
Anim Reprod Sci. 2016 Oct;173:97-103. doi: 10.1016/j.anireprosci.2016.09.002. Epub 2016 Sep 4.
Two trials were conducted to evaluate the effect of diverse socio-sexual cues upon male sexual behavior and the reproductive performance of anestrous does (AD). Trials were conducted in northern Mexico (26°N) during the natural anestrous season (Feb-Mar) with crossbred dairy bucks. In Experiment 1, sexually inactive bucks (SIB, n=12) were randomly allotted to three groups, four males/group: a) DEE 9novelty stimulation) - daily exchange of estrogenized females (EF) 12&12h, b) NEE (no novelty stimulation) - no-exchange of EF, 24h, or c) CON (saline-treated_ -daily exchange of AF 12&12h. Sexually active bucks (SAB) from the DEE, NEE and CON groups were subsequently exposed to AD (n=72; n=24/group) and the reproductive outcomes were recorded. In Experiment 2, SAB (n=12; n=6/group) were randomly divided in: 1) B+EF - males+four-EF exposed to AF (n=36), and 2) B+NEF; males+four-saline-treated AD and exposed to AD (n=36). Prior to the onset of the experimental breeding in both experiments, the investigative (ISB), consummatory (CSB) and resting (RSB) sexual behavior of males were quantified (2h×d×2d). Sexual behaviors considered were: ISB - flehmen, ano-genital sniffing, approaches, vocalizations, kicking, penis extrusion, CSB; mount attempts and mounts, and RSB - isolation, attempted escape, aggression and distractions. While EF were an effective stimulus (P<0.05) for evoking mounting in SIB males, daily exchange of estrous does used to stimulate males promoted an enhanced response (P<0.05) in terms of both ISB and CSB. After being exposed to AD, the B+EF bucks induced an earlier estrous response (P<0.05) as compared with the B+NEF bucks. The untreated females did not induce any sexual activity in males and stimulation of ovarian function did not occur when saline treated (CON) AD were exposed to AD. Also, the B+EF group induced an enhanced increase (P<0.05) of the male ISB and CSB, inducing in turn an increase percentage onset of estrus in does that had previously been anestrus (P<0.05).
进行了两项试验，以评估不同的社会性行为线索对雄性性行为和处于乏情期母羊（AD）繁殖性能的影响。试验在墨西哥北部（北纬26°）自然乏情季节（2月至3月）使用杂交奶山羊进行。在实验1中，性不活跃的公羊（SIB，n = 12）被随机分为三组，每组四只公羊：a）DEE（新颖刺激）——每天12小时和12小时交替更换雌激素处理的母羊（EF）；b）NEE（无新颖刺激）——不更换EF，持续24小时；或c）CON（生理盐水处理）——每天12小时和12小时交替更换未处理的母羊（AF）。随后，将来自DEE、NEE和CON组的性活跃公羊（SAB）与AD母羊（n = 72；每组n = 24）接触，并记录繁殖结果。在实验2中，SAB（n = 12；每组n = 6）被随机分为：1）B + EF组——公羊 + 四只EF母羊接触AF母羊（n = 36），以及2）B + NEF组——公羊 + 四只生理盐水处理的AD母羊并接触AD母羊（n = 36）。在两个实验的实验性配种开始前，对雄性的探究性行为（ISB）、交配性行为（CSB）和休息性行为（RSB）进行了量化（每天2小时，共2天）。所考虑的性行为包括：ISB——卷唇、肛门 - 生殖器嗅闻、接近、发声、踢、阴茎伸出；CSB——爬跨尝试和爬跨；以及RSB——隔离、试图逃跑、攻击和分心。虽然EF是激发SIB雄性爬跨的有效刺激（P < 0.05），但用于刺激雄性的发情母羊的每日更换在ISB和CSB方面促进了更强的反应（P < 0.05）。在接触AD母羊后，与B + NEF组公羊相比，B + EF组公羊诱导出更早的发情反应（P < 0.05）。未处理的母羊在雄性中未诱发任何性活动，并且当生理盐水处理的（CON）AD母羊与AD母羊接触时，卵巢功能未被刺激。此外，B + EF组诱导雄性ISB和CSB增强增加（P < 0.05），进而使先前处于乏情期的母羊发情开始百分比增加（P < 0.05）。
