Wang Juan, Wang Yang, Liu Xinjie, Xu Yuanliu, Ma Qing
State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, China.
Biomed Res Int. 2016;2016:7830768. doi: 10.1155/2016/7830768. Epub 2016 Aug 16.
The plant cytoskeleton, including microtubules and microfilaments, is one of the important factors in determining the polarity of cell division and growth, as well as the interaction of plants with invading pathogens. In defense responses of wheat against the stripe rust (Puccinia striiformis f. sp. tritici) infection, hypersensitive response is the most crucial event to prevent the spread of pathogens. In order to reveal the effect of microtubules on the hypersensitive cell death and H2O2 accumulation in the interaction of wheat (Triticum aestivum) cv. Suwon 11 with an incompatible race, CYR23, wheat leaves were treated with microtubule inhibitor, oryzalin, before inoculation. The results showed that the frequency of infection sites with hypersensitive response occurrence was significantly reduced, and hypersensitive cell death in wheat leaves was suppressed compared to the control. In addition, the frequency and the incidence of infected cells with H2O2 accumulation were also reduced after the treatment with oryzalin. Those results indicated that microtubules are related to hypersensitive response and H2O2 accumulation in wheat induced by the stripe rust, and depolymerization of microtubules reduces the resistance of plants to pathogen infection in incompatible interaction, suggesting that microtubules play a potential role in the expression of resistance of wheat against the stripe rust fungus.
植物细胞骨架，包括微管和微丝，是决定细胞分裂和生长极性以及植物与入侵病原体相互作用的重要因素之一。在小麦对条锈病（条形柄锈菌小麦专化型）感染的防御反应中，过敏反应是阻止病原体传播的最关键事件。为了揭示微管对小麦品种苏旺11与不亲和小种CYR23互作中过敏细胞死亡和H2O2积累的影响，在接种前用微管抑制剂oryzalin处理小麦叶片。结果表明，与对照相比，出现过敏反应的感染位点频率显著降低，小麦叶片中的过敏细胞死亡受到抑制。此外，用oryzalin处理后，积累H2O2的感染细胞频率和发生率也降低了。这些结果表明，微管与条锈菌诱导的小麦过敏反应和H2O2积累有关，微管解聚降低了不亲和互作中植物对病原体感染的抗性，表明微管在小麦对条锈菌抗性表达中发挥潜在作用。
