Saha S K, Saha S, Akhter S M, Khatun S, Islam M M, Roy P
Dr Shyamal Kumar Saha, Associate Professor & Head, Department of Pharmacology & Therapeutics, Mymensingh Medical College (MMC), Mymensingh, Bangladesh.
Mymensingh Med J. 2016 Jul;25(3):477-84.
An interventional study was performed to determine and compare the MICs of aqueous garlic extract (AGE) and Imipenem against standard strains of Staphylococcus aureus ATCC 25923 & Eschericha coli ATCC 25922. The study was conducted in Department of Pharmacology and Therapeutics in collaboration with Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh from July 2014 to January 2015. The MIC of AGE and antibiotic Imipenem were determined with the help of broth dilution method. The MIC of AGE was determined as 400μg/ml and 700μg/ml against Staphylococcus aureus, and Escherichia coli respectively and the MIC of Imipenem was 1μg/ml against Staphylococus aureus and 1.5μg/ml against Escherichia coli. The MICs of Imipenem was much lower in comparison to MICs of AGE for the test organisms. The subculture study showed the same results with that of the primary isolates. From the study it was clearly observed that AGE have anti bacterial effect but is not potent like antibiotic Imipenem. In this regard active ingredient present in garlic needs to be separated & purified for further study.
进行了一项干预性研究，以测定并比较大蒜水提取物（AGE）和美罗培南对金黄色葡萄球菌ATCC 25923和大肠杆菌ATCC 25922标准菌株的最低抑菌浓度（MIC）。该研究于2014年7月至2015年1月在孟加拉国迈门辛医学院药理学与治疗学系与微生物学系合作开展。借助肉汤稀释法测定了AGE和美罗培南的MIC。AGE对金黄色葡萄球菌和大肠杆菌的MIC分别测定为400μg/ml和700μg/ml，美罗培南对金黄色葡萄球菌的MIC为1μg/ml，对大肠杆菌的MIC为1.5μg/ml。对于受试微生物，美罗培南的MIC比AGE的MIC低得多。传代培养研究显示的结果与原始分离株相同。从该研究中清楚地观察到，AGE具有抗菌作用，但不像抗生素美罗培南那样强效。在这方面，大蒜中存在的活性成分需要分离和纯化以供进一步研究。
