Brankov Nikoleta, Prodanovic Edward M, Hurley M Yadira
Department of Medicine, Loma Linda University, Loma Linda, CA, USA.
Department of Dermatology, Private Practice, Huntington Beach, CA, USA.
J Cutan Pathol. 2016 Dec;43(12):1139-1142. doi: 10.1111/cup.12819. Epub 2016 Oct 4.
Studies on the precise cause of increased melanization in pigmented basal cell carcinomas (BCC) are limited. We aimed to determine whether the cause of melanization is from increased number of melanocytes or increased melanin pigment, and if there is a difference in the number of melanocytes on different sun-exposed locations.
A retrospective review of 45 skin biopsies from January 2011 to February 2011 was performed; 30 were diagnosed as pigmented BCC and 15 as non-pigmented BCC. Immunohistochemistry for MART-1 (melanoma-associated antigen recognized by T-cell 1)/Melan-A (clone M2-7610 + M2-9E3; Leica Microsystems Inc. Buffalo Grove, IL, USA) from Biocare Medical (Concord, CA, USA) was performed on all biopsies. Associations between histopathologic features, number of melanocytes, location, and specific diagnoses were analyzed by Mann-Whitney U test.
The mean melanocyte count per high powered field in pigmented BCCs from sun-exposed skin was 101.9 and from intermittently sun-exposed skin was 122.5, as compared to the controls (nodular non-pigmented BCC) of 27.4 (p = 0.002) and 34.9 (p = 0.002), respectively.
Pigmented BCCs have a higher mean melanocyte count as compared to non-pigmented BCCs irrespective of location. Therefore, the pigment is not only due to increased melanin, but also due to increased melanocytes.
关于色素性基底细胞癌（BCC）中黑素沉着增加的确切原因的研究有限。我们旨在确定黑素沉着的原因是黑素细胞数量增加还是黑色素色素增加，以及在不同阳光暴露部位的黑素细胞数量是否存在差异。
对2011年1月至2011年2月的45例皮肤活检进行回顾性研究；30例被诊断为色素性BCC，15例为非色素性BCC。对所有活检组织进行来自美国加利福尼亚州康科德市Biocare Medical公司的MART-1（T细胞识别的黑色素瘤相关抗原1）/Melan-A（克隆M2-7610 + M2-9E3；美国伊利诺伊州布法罗格罗夫市徕卡微系统公司）免疫组织化学检测。通过Mann-Whitney U检验分析组织病理学特征、黑素细胞数量、部位和具体诊断之间的关联。
暴露于阳光的皮肤色素性BCC每高倍视野的平均黑素细胞计数为101.9，间歇性暴露于阳光的皮肤为122.5，而对照组（结节性非色素性BCC）分别为27.4（p = 0.002）和34.9（p = 0.002）。
无论部位如何，色素性BCC的平均黑素细胞计数均高于非色素性BCC。因此，色素不仅归因于黑色素增加，还归因于黑素细胞增加。
