Liu Wenjie, Li Huiyuan, Hao Yating, Li Yang, Lv Mingen, Xue Feng, Liu Xiaofan, Zhang Lei, Yang Renchi
State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China; Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China.
State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.
Mol Immunol. 2016 Oct;78:89-97. doi: 10.1016/j.molimm.2016.08.014. Epub 2016 Sep 7.
Primary immune thrombocytopenia (ITP) is an acquired autoimmune disease. 1α, 25-dihydroxyvitamin D [1,25(OH)D] and vitamin D receptor (VDR) play important immune-suppressive roles in immune system. It has been reported that serum 1,25(OH)D were lower in ITP patients. In this study, we evaluated local 1,25(OH)D level and VDR mRNA expression further, and determined whether 1,25(OH)D/VDR were correlated with T cell dysfunction in ITP patients. We found that 1,25(OH)D/VDR levels were decreased in active ITP patients, and 1,25(OH)D had significant anti-inflammatory effects on ITP patients, including both anti-proliferation of peripheral blood mononuclear cells (PBMCs) and reversing the abnormal T cells polarization. 1,25(OH)D inhibited the differentiation of T helper (Th)1 and Tc1 cells but induced the differentiation of Th2, Tc2 and T regulatory (Treg) cells in ITP patients. However, the percentage of Th17 cells were not affected obviously with 1,25(OH)D. In addition, 1,25(OH)D also suppressed pro-inflammatory cytokines (INF-γ and IL-17A) but promoted anti-inflammatory cytokine (IL-10) secretion in ITP patients. In conclusion, decreased 1,25(OH)D/VDR might participate in the pathogenesis of ITP, and appropriate supplement of 1,25(OH)D may be a promising treatment.
原发性免疫性血小板减少症（ITP）是一种获得性自身免疫性疾病。1α,25 - 二羟基维生素D [1,25(OH)D] 和维生素D受体（VDR）在免疫系统中发挥重要的免疫抑制作用。据报道，ITP患者的血清1,25(OH)D水平较低。在本研究中，我们进一步评估了局部1,25(OH)D水平和VDR mRNA表达，并确定1,25(OH)D/VDR是否与ITP患者的T细胞功能障碍相关。我们发现，活动期ITP患者的1,25(OH)D/VDR水平降低，并且1,25(OH)D对ITP患者具有显著的抗炎作用，包括对外周血单个核细胞（PBMCs）的抗增殖作用以及逆转异常的T细胞极化。1,25(OH)D抑制ITP患者中辅助性T（Th）1细胞和细胞毒性T（Tc）1细胞的分化，但诱导Th2、Tc2和调节性T（Treg）细胞的分化。然而，1,25(OH)D对Th17细胞的百分比没有明显影响。此外，1,25(OH)D还抑制ITP患者中促炎细胞因子（INF - γ和IL - 17A）的分泌，但促进抗炎细胞因子（IL - 10）的分泌。总之，1,25(OH)D/VDR降低可能参与ITP的发病机制，适当补充1,25(OH)D可能是一种有前景的治疗方法。
