Medina-Puche Laura, Blanco-Portales Rosario, Molina-Hidalgo Francisco Javier, Cumplido-Laso Guadalupe, García-Caparrós Nicolás, Moyano-Cañete Enriqueta, Caballero-Repullo José Luis, Muñoz-Blanco Juan, Rodríguez-Franco Antonio
Departamento de Bioquímica y Biología Molecular. Edificio Severo Ochoa, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, 14071, Córdoba, Spain.
Funct Integr Genomics. 2016 Nov;16(6):671-692. doi: 10.1007/s10142-016-0510-3. Epub 2016 Sep 10.
Strawberry is an ideal model for studying the molecular biology of the development and ripening of non-climacteric fruits. Hormonal regulation of gene expression along all these processes in strawberries is still to be fully elucidated. Although auxins and ABA have been pointed out as the major regulatory hormones, few high-throughput analyses have been carried out to date. The role for ethylene and gibberellins as regulatory hormones during the development and ripening of the strawberry fruit remain still elusive. By using a custom-made and high-quality oligo microarray platform done with over 32,000 probes including all of the genes actually described in the strawberry genome, we have analysed the expression of genes during the development and ripening in the receptacles of these fruits. We classify these genes into two major groups depending upon their temporal and developmental expression. First group are genes induced during the initial development stages. The second group encompasses genes induced during the final maturation and ripening processes. Each of these two groups has been also divided into four sub-groups according their pattern of hormonal regulation. By analyzing gene expression, we clearly show that auxins and ABA are the main and key hormones that combined or independently are responsible of the development and ripening process. Auxins are responsible for the receptacle fruit development and, at the same time¸ prevent ripening by repressing crucial genes. ABA regulates the expression of the vast majority of genes involved in the ripening. The main genes expressed under the control of these hormones are presented and their physiological rule discussed. We also conclude that ethylene and gibberellins do not seem to play a prominent role during these processes.
草莓是研究非跃变型果实发育和成熟分子生物学的理想模型。草莓果实发育和成熟过程中激素对基因表达的调控仍有待充分阐明。尽管生长素和脱落酸已被指出是主要的调控激素，但迄今为止很少进行高通量分析。乙烯和赤霉素在草莓果实发育和成熟过程中作为调控激素的作用仍不清楚。通过使用定制的高质量寡核苷酸微阵列平台，该平台包含超过32000个探针，涵盖了草莓基因组中实际描述的所有基因，我们分析了这些果实花托在发育和成熟过程中的基因表达。根据基因的时空表达和发育情况，我们将这些基因分为两大类。第一类是在初始发育阶段诱导表达的基因。第二类包括在最终成熟和成熟过程中诱导表达的基因。这两类基因又根据其激素调控模式分为四个亚类。通过分析基因表达，我们清楚地表明，生长素和脱落酸是主要且关键的激素，它们共同或独立地负责果实的发育和成熟过程。生长素负责花托果实的发育，同时通过抑制关键基因来阻止果实成熟。脱落酸调控绝大多数参与成熟的基因的表达。我们展示了在这些激素控制下表达的主要基因，并讨论了它们的生理作用。我们还得出结论，乙烯和赤霉素在这些过程中似乎不发挥突出作用。
