采用多壁碳纳米管的人脂联素-2 的快速高灵敏免疫分析方法。
A rapid and highly sensitive immunoassay format for human lipocalin-2 using multiwalled carbon nanotubes.
机构信息
Institute of Clinical Chemistry and Pathobiochemistry, Klinikum rechts der Isar der Technischen Universität München, Ismaninger Str. 22, Munich d-81675, Germany; Vallo Med Health Care GmbH, Bahnhofstr. 200, Castrop-Rauxel 44579, Germany.
Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), Department of Chemistry and Analytical, Biological Chemistry Research Facility (ABCRF), University College Cork, Cork, Ireland.
出版信息
Biosens Bioelectron. 2017 Jul 15;93:198-204. doi: 10.1016/j.bios.2016.09.010. Epub 2016 Sep 3.
A sensitive and rapid sandwich immunoassay (IA) was developed for human lipocalin-2 (LCN2) by functionalizing a KOH-treated polystyrene microtiter plate with multiwalled carbon nanotubes (MWCNTs) dispersed in 3-aminoproyltriethoxysilane (APTES). The significantly increased surface area due to the presence of MWCNTs led to a high immobilization density of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) activated anti-LCN2 capture antibodies (Ab). The anti-LCN2 Ab-bound MTPs were stable for 6 weeks when stored in 0.1M PBS, pH 7.4 at 4°C. The IA detects LCN2 from 0.6 to 5120pgmL with a limit of detection (LOD) and limit of quantification (LOQ) of 0.9pgmL and 6pgmL, respectively. The assay offered a ~50-fold lower LOD and ~3-fold faster IA, compared to a commercial sandwich enzyme-linked immunosorbent assay kit.
一种敏感且快速的夹心免疫分析（IA）通过在经 KOH 处理的聚苯乙烯微量滴定板上功能化多壁碳纳米管（MWCNTs）在 3-氨基丙基三乙氧基硅烷（APTES）中分散来检测人脂联素-2（LCN2）。由于 MWCNTs 的存在，表面积显著增加，导致 1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）激活的抗 LCN2 捕获抗体（Ab）的固定密度很高。当在 4°C 的 0.1M PBS、pH 7.4 中储存时，结合 Ab 的 MTP 稳定 6 周。IA 可检测 0.6 至 5120pgmL 的 LCN2，检测限（LOD）和定量限（LOQ）分别为 0.9pgmL 和 6pgmL。与商业夹心酶联免疫吸附测定试剂盒相比，该测定法的 LOD 降低约 50 倍，IA 快约 3 倍。
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