Kumari T S, Vaidyanath K
Department of Genetics, Osmania University, Hyderabad, India.
Mutat Res. 1989 Aug;226(4):235-8. doi: 10.1016/0165-7992(89)90075-4.
The mutagenic activity of 2,4-D was assessed utilising cytogenetic, chlorophyll mutation, specific-locus and pollen viability endpoints in Allium cepa and Oryza sativa. In the Allium root-tip assay onion bulbs with roots were treated with 25, 50, 75 or 100 ppm 2,4-D for 4 h and the frequency of aberrations was estimated. With an increase in concentration there was rise in frequency of aberrant cells. In the case of mutation assays, presoaked rice seeds were treated with 100, 200 or 300 ppm 2,4-D for 4 h and sown in the field. Mutation frequency was estimated on M1 plant, spike and M2 seedling basis. With increasing concentration, there was a concomitant increase in mutation frequency. Highly significant chlorophyll-deficient and waxy mutants besides sterile pollen were observed as compared to both positive (EMS) and negative controls (H2O). The study demonstrates that 2,4-D has genotoxic potential.
利用洋葱（Allium cepa）和水稻（Oryza sativa）的细胞遗传学、叶绿素突变、特定位点和花粉活力等指标，评估了2,4-二氯苯氧乙酸（2,4-D）的诱变活性。在洋葱根尖试验中，将带有根的洋葱鳞茎用25、50、75或100 ppm的2,4-D处理4小时，然后估算畸变频率。随着浓度增加，异常细胞的频率升高。在突变试验中，将预先浸泡的水稻种子用100、200或300 ppm的2,4-D处理4小时，然后播种到田间。基于M1植株、穗和M2幼苗估算突变频率。随着浓度增加，突变频率随之升高。与阳性对照（甲基磺酸乙酯，EMS）和阴性对照（水）相比，观察到了大量显著的叶绿素缺乏和蜡质突变体以及不育花粉。该研究表明2,4-D具有遗传毒性潜力。
