自由活动条件下大鼠额叶皮质中胆囊收缩素样免疫反应性的体内释放。

In vivo release of cholecystokinin-like immunoreactivity in rat frontal cortex under freely moving conditions.

作者信息

Takita M, Tsuruta T, Oh-hashi Y, Kato T

机构信息

Department of Life Chemistry, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

Neurosci Lett. 1989 May 22;100(1-3):249-53. doi: 10.1016/0304-3940(89)90693-9.

DOI:10.1016/0304-3940(89)90693-9

PMID:2761773

Abstract

Release of cholecystokinin-like immunoreactivity (CCK-LI) was measured in rat medial prefrontal cortex in vivo by brain dialysis and enzyme immunoassay under freely moving condition. Perfusion of 50 mM K+ resulted in the increase of CCK-LI in the dialysate. The data on high-performance liquid chromatography (HPLC) of the dialysate showed that the increase of the CCK-LI was mainly due to the increase of CCK octapeptide sulfate itself. In the preliminary experiments, we applied this brain dialysis method for determination of CCK-LI release with drug treatment. After treatment with sulpiride (i.p.), a D2 dopamine receptor antagonist, a significant increase of CCK-LI was observed, indicating that this brain dialysis technique is applicable to detect change in the level of CCK-LI release after a certain drug treatment.

摘要

在自由活动条件下，通过脑透析和酶免疫测定法在大鼠内侧前额叶皮质体内测量胆囊收缩素样免疫反应性（CCK-LI）的释放。灌注50 mM K+导致透析液中CCK-LI增加。透析液的高效液相色谱（HPLC）数据表明，CCK-LI的增加主要是由于硫酸CCK八肽本身的增加。在初步实验中，我们应用这种脑透析方法来测定药物治疗时CCK-LI的释放。在用D2多巴胺受体拮抗剂舒必利（腹腔注射）治疗后，观察到CCK-LI显著增加，表明这种脑透析技术适用于检测特定药物治疗后CCK-LI释放水平的变化。