Mashtoub Suzanne, Lampton Lorrinne S, Eden Georgina L, Cheah Ker Y, Lymn Kerry A, Bajic Juliana E, Howarth Gordon S
a School of Medicine and Pharmacology, The University of Western Australia, Harry Perkins Institute for Medical Research, Fiona Stanley Hospital , Murdoch , Western Australia.
b Department of Gastroenterology , Women's and Children's Hospital , North Adelaide , South Australia.
Nutr Cancer. 2016 Oct;68(7):1171-80. doi: 10.1080/01635581.2016.1208829. Epub 2016 Aug 11.
Chemotherapy-induced mucositis is characterized by inflammation and ulcerating lesions lining the alimentary tract. Emu Oil and Lyprinol™ have independently demonstrated their therapeutic potential in intestinal inflammatory disorders, including mucositis. We investigated Emu Oil and Lyprinol™ in combination for their further potential to alleviate chemotherapy-induced mucositis in rats. Rats were gavaged with (1 ml) water, Olive Oil, Emu Oil + Olive Oil, Lyprinol™ + Olive Oil or Emu Oil + Lyprinol™ from Days 0 to 7, injected with saline (control) or 5-Fluorouracil (5-FU) on Day 5 and euthanized on Day 8. Myeloperoxidase (MPO) activity (indicative of acute inflammation), histological severity scores, and intestinal architecture were quantified. Myeloperoxidase activity was significantly increased in the jejunum and ileum following 5-FU, compared to saline controls. Both Olive Oil and Emu Oil + Lyprinol™ significantly reduced jejunal MPO levels (1.8-fold and 1.7-fold, respectively), whereas only Emu Oil + Lyprinol™ significantly decreased ileal MPO levels, relative to 5-FU controls. All oil treatments decreased histological severity scores in the jejunum and ileum, and normalized crypt depth in the mid small intestine, relative to 5-FU controls. Emu Oil combined with Lyprinol™ partially reduced acute small intestinal inflammation. Isolating bioactive constituents of these naturally sourced oils could provide a more targeted strategy to protect against intestinal mucositis.
化疗引起的粘膜炎的特征是消化道出现炎症和溃疡性病变。鸸鹋油和Lyprinol™已分别证明它们在包括粘膜炎在内的肠道炎症性疾病中的治疗潜力。我们研究了鸸鹋油和Lyprinol™联合使用对减轻大鼠化疗引起的粘膜炎的进一步潜力。从第0天到第7天，给大鼠灌胃（1毫升）水、橄榄油、鸸鹋油+橄榄油、Lyprinol™+橄榄油或鸸鹋油+Lyprinol™，在第5天注射生理盐水（对照）或5-氟尿嘧啶（5-FU），并在第8天实施安乐死。对髓过氧化物酶（MPO）活性（指示急性炎症）、组织学严重程度评分和肠道结构进行了量化。与生理盐水对照组相比，5-FU处理后空肠和回肠中的髓过氧化物酶活性显著增加。相对于5-FU对照组，橄榄油和鸸鹋油+Lyprinol™均显著降低了空肠MPO水平（分别降低了1.8倍和1.7倍），而只有鸸鹋油+Lyprinol™显著降低了回肠MPO水平。相对于5-FU对照组，所有油处理均降低了空肠和回肠的组织学严重程度评分，并使小肠中部的隐窝深度恢复正常。鸸鹋油与Lyprinol™联合使用可部分减轻急性小肠炎症。分离这些天然来源油中的生物活性成分可能提供一种更具针对性的策略来预防肠道粘膜炎。
