Niwayama Satomi, Zabet-Moghaddam Masoud, Kurono Sadamu, Kattanguru Pullaiah, Shaikh Aarif L
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA; Department of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA; Graduate School of Engineering, Muroran Institute of Technology, Muroran, Hokkaido 050-8585, Japan.
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA.
Bioorg Med Chem Lett. 2016 Oct 15;26(20):5073-5077. doi: 10.1016/j.bmcl.2016.08.079. Epub 2016 Aug 24.
Ethyl succinic anhydride and its d-labeled version have been synthesized and applied to quantitative analysis of peptides in combination with MALDI or ESI mass spectrometry. These modifiers react with amino groups in the N-termini and lysine side chains in proteins, and therefore the combination of these modifiers was shown to be a useful tool for quantification of peptides and hence for proteomics research.
乙基琥珀酸酐及其氘标记版本已被合成，并与基质辅助激光解吸电离（MALDI）或电喷雾电离（ESI）质谱联用应用于肽的定量分析。这些修饰剂与蛋白质N端的氨基和赖氨酸侧链发生反应，因此这些修饰剂的组合被证明是定量肽的有用工具，从而也可用于蛋白质组学研究。
