氘标记和未标记的乙基琥珀酸酐的合成及其在肽的同位素示差质谱定量分析中的应用。
Synthesis of d-labeled and unlabeled ethyl succinic anhydrides and application to quantitative analysis of peptides by isotope differential mass spectrometry.
作者信息
Niwayama Satomi, Zabet-Moghaddam Masoud, Kurono Sadamu, Kattanguru Pullaiah, Shaikh Aarif L
机构信息
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA; Department of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA; Graduate School of Engineering, Muroran Institute of Technology, Muroran, Hokkaido 050-8585, Japan.
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA.
出版信息
Bioorg Med Chem Lett. 2016 Oct 15;26(20):5073-5077. doi: 10.1016/j.bmcl.2016.08.079. Epub 2016 Aug 24.
Ethyl succinic anhydride and its d-labeled version have been synthesized and applied to quantitative analysis of peptides in combination with MALDI or ESI mass spectrometry. These modifiers react with amino groups in the N-termini and lysine side chains in proteins, and therefore the combination of these modifiers was shown to be a useful tool for quantification of peptides and hence for proteomics research.
乙基琥珀酸酐及其氘标记版本已被合成，并与基质辅助激光解吸电离（MALDI）或电喷雾电离（ESI）质谱联用应用于肽的定量分析。这些修饰剂与蛋白质N端的氨基和赖氨酸侧链发生反应，因此这些修饰剂的组合被证明是定量肽的有用工具，从而也可用于蛋白质组学研究。
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